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protein dna_rna metals Protein-protein interface(s) links
Isomerase/RNA PDB id
2rfk
Jmol
Contents
Protein chains
334 a.a. *
53 a.a. *
74 a.a. *
DNA/RNA
Metals
_ZN
* Residue conservation analysis
PDB id:
2rfk
Name: Isomerase/RNA
Title: Substrate RNA positioning in the archaeal h/aca ribonucleoprotein complex
Structure: Probable tRNA pseudouridine synthase b. Chain: a. Synonym: tRNA pseudouridine 55 synthase, psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase. Engineered: yes. Mutation: yes. Ribosome biogenesis protein nop10. Chain: b. Engineered: yes.
Source: Pyrococcus furiosus. Organism_taxid: 2261. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Resolution:
2.87Å     R-factor:   0.250     R-free:   0.300
Authors: B.Liang,S.Xue,R.M.Terns,M.P.Terns,H.Li,Southeast Collaboratory For Structural Genomics (Secsg)
Key ref: B.Liang et al. (2007). Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex. Nat Struct Mol Biol, 0, 0. PubMed id: 18059286 DOI: 10.1038/nsmb1336
Date:
30-Sep-07     Release date:   05-Feb-08    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q7LWY0  (TRUB_PYRFU) -  Probable tRNA pseudouridine synthase B
Seq:
Struc: 		340 a.a.
334 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8U1R4  (NOP10_PYRFU) -  Ribosome biogenesis protein Nop10
Seq:
Struc: 		60 a.a.
53 a.a.
Protein chain
Pfam   ArchSchema ?
Q8U029  (Q8U029_PYRFU) -  Small nucleolar rnp gar1-like protein
Seq:
Struc: 		104 a.a.
74 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chain A: E.C.5.4.99.25  - tRNA pseudouridine(55) synthase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: tRNA uridine55 = tRNA pseudouridine55
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     box H/ACA snoRNP complex   1 term 
  Biological process     ribosome biogenesis   6 terms 
  Biochemical function     isomerase activity     4 terms  

 

 
DOI no: 10.1038/nsmb1336 Nat Struct Mol Biol 0:0 (2007)
PubMed id: 18059286  
 
 
Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex.
B.Liang, S.Xue, R.M.Terns, M.P.Terns, H.Li.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19744324 J.Donovan, and P.R.Copeland (2009).
Evolutionary history of selenocysteine incorporation from the perspective of SECIS binding proteins.
  BMC Evol Biol, 9, 229.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.