PDBsum entry: 2qme

Signaling protein/transferase

PDB id: 2qme

Contents

Protein chains

178 a.a. *

24 a.a. *

Ligands

GCP

GOL ×2

Metals

_MG

Waters ×238

* Residue conservation analysis

PDB id:

2qme

Name:

Signaling protein/transferase

Title:

Crystal structure of human rac3 in complex with crib domain p21-activated kinase 1 (pak1)

Structure:

Ras-related c3 botulinum toxin substrate 3. Chain: a. Synonym: p21-rac3. Engineered: yes. Crib domain of the serine/threonine-protein kinas chain: i. Fragment: crib domain. Synonym: p21-activated kinase 1, pak-1, p65-pak, alpha-pak. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rac3. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic peptide with the sequence of thE C domain of human pak1 protein

Resolution:

1.75Å R-factor: 0.151 R-free: 0.192

Authors:

E.Ugochukwu,X.Yang,J.M.Elkins,N.Burgess-Brown,G.Bunkoczi,M.S C.H.Arrowsmith,J.Weigelt,A.Edwards,F.Von Delft,S.Knapp,D.Do Structural Genomics Consortium (Sgc)

Key ref:

E.Ugochukwu et al. The crystal structure of the human rac3 in complex with the crib domain of human p21-Activated kinase 1 (pak1).. To be published,

Date:

16-Jul-07 Release date: 28-Aug-07

Protein chain

P60763  (RAC3_HUMAN) -  Ras-related C3 botulinum toxin substrate 3

Seq: 192 a.a.

Struc: 178 a.a.*

Protein chain

Q13153  (PAK1_HUMAN) -  Serine/threonine-protein kinase PAK 1

Seq: 545 a.a.

Struc: 24 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain I: E.C.2.7.11.1 - Non-specific serine/threonine protein kinase.
• Reaction: ATP + a protein = ADP + a phosphoprotein

ATP + protein = ADP (Bound ligand (Het Group name = GCP) matches with 78.79% similarity) + phosphoprotein

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: intracellular (12 terms)
• Biological process: intracellular signal transduction (9 terms)
• Biochemical function: nucleotide binding (4 terms)