PDBsum entry: 2pxz

Lyase

PDB id: 2pxz

Contents

Protein chain

526 a.a. *

Ligands

OAA

ATP

CO2

Metals

_MG

_MN

Waters ×283

* Residue conservation analysis

PDB id:

2pxz

Name:

Lyase

Title:

E. Coli phosphoenolpyruvate carboxykinase (pepck) complexed mg2+, mn2+, carbon dioxide and oxaloacetate

Structure:

Phosphoenolpyruvate carboxykinase. Chain: x. Synonym: pep carboxykinase, phosphoenolpyruvate carboxylase engineered: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: pcka. Expressed in: escherichia coli k12. Expression_system_taxid: 83333.

Resolution:

2.23Å R-factor: 0.176 R-free: 0.234

Authors:

L.T.J.Delbaere,J.J.H.Cotelesage,H.Goldie

Key ref:

L.T.J.Delbaere et al. Crystal structure of e. Coli phosphoenolpyruvate carboxykinase (pepck) complexed with atp, Mg2+, Mn2+, Carbon dioxide and oxaloacetate. To be published,

Date:

14-May-07 Release date: 22-Apr-08

Protein chain

P22259  (PCKA_ECOLI) -  Phosphoenolpyruvate carboxykinase [ATP]

Seq: 540 a.a.

Struc: 526 a.a.

Enzyme reactions

• Enzyme class: E.C.4.1.1.49 - Phosphoenolpyruvate carboxykinase (ATP).
• Reaction: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO₂

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + oxaloacetate (Bound ligand (Het Group name = OAA) corresponds exactly) = ADP + phosphoenolpyruvate + CO(2) (Bound ligand (Het Group name = CO2) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: cytoplasm (1 term)
• Biological process: metabolic process (2 terms)
• Biochemical function: catalytic activity (8 terms)