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PDBsum entry 2pf4

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protein metals Protein-protein interface(s) links
Hydrolase regulator/viral protein PDB id
2pf4
Jmol
Contents
Protein chains
575 a.a. *
163 a.a. *
152 a.a. *
142 a.a. *
Metals
_ZN ×8
* Residue conservation analysis
PDB id:
2pf4
Name: Hydrolase regulator/viral protein
Title: Crystal structure of the full-length simian virus 40 small t complexed with the protein phosphatase 2a aalpha subunit
Structure: Serine/threonine-protein phosphatase 2a 65 kda re subunit a alpha isoform. Chain: a, b, c, d. Synonym: pp2a, subunit a, pr65-alpha isoform, pp2a, subunit alpha isoform. Engineered: yes. Small t antigen. Chain: e, f, g, h. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: ppp2r1a. Expressed in: escherichia coli. Expression_system_taxid: 562. Simian virus 40. Organism_taxid: 10633. Strain: va45-54-2.
Resolution:
3.10Å     R-factor:   0.247     R-free:   0.304
Authors: U.Cho,S.Morrone,W.Xu
Key ref: U.S.Cho et al. (2007). Structural basis of PP2A inhibition by small t antigen. PLoS Biol, 5, e202. PubMed id: 17608567
Date:
03-Apr-07     Release date:   07-Aug-07    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q76MZ3  (2AAA_MOUSE) -  Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Seq:
Struc:
 
Seq:
Struc:
589 a.a.
575 a.a.
Protein chain
Pfam   ArchSchema ?
P03081  (ST_SV40) -  Small t antigen
Seq:
Struc:
174 a.a.
163 a.a.*
Protein chain
Pfam   ArchSchema ?
P03081  (ST_SV40) -  Small t antigen
Seq:
Struc:
174 a.a.
152 a.a.*
Protein chains
Pfam   ArchSchema ?
P03081  (ST_SV40) -  Small t antigen
Seq:
Struc:
174 a.a.
142 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein phosphatase type 2A complex   7 terms 
  Biological process     positive regulation of extrinsic apoptotic signaling pathway in absence of ligand   8 terms 
  Biochemical function     antigen binding     5 terms  

 

 
PLoS Biol 5:e202 (2007)
PubMed id: 17608567  
 
 
Structural basis of PP2A inhibition by small t antigen.
U.S.Cho, S.Morrone, A.A.Sablina, J.D.Arroyo, W.C.Hahn, W.Xu.
 
  ABSTRACT  
 
The SV40 small t antigen (ST) is a potent oncoprotein that perturbs the function of protein phosphatase 2A (PP2A). ST directly interacts with the PP2A scaffolding A subunit and alters PP2A activity by displacing regulatory B subunits from the A subunit. We have determined the crystal structure of full-length ST in complex with PP2A A subunit at 3.1 A resolution. ST consists of an N-terminal J domain and a C-terminal unique domain that contains two zinc-binding motifs. Both the J domain and second zinc-binding motif interact with the intra-HEAT-repeat loops of HEAT repeats 3-7 of the A subunit, which overlaps with the binding site of the PP2A B56 subunit. Intriguingly, the first zinc-binding motif is in a position that may allow it to directly interact with and inhibit the phosphatase activity of the PP2A catalytic C subunit. These observations provide a structural basis for understanding the oncogenic functions of ST.