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PDBsum entry 2opo

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protein ligands metals Protein-protein interface(s) links
Allergen PDB id
2opo
Jmol
Contents
Protein chains
81 a.a. *
85 a.a. *
Ligands
SO4 ×4
Metals
_CA ×8
Waters ×292
* Residue conservation analysis
PDB id:
2opo
Name: Allergen
Title: Crystal structure of the calcium-binding pollen allergen che
Structure: Polcalcin che a 3. Chain: a, b, c, d. Synonym: calcium-binding pollen allergen che a 3. Engineered: yes
Source: Chenopodium album. Organism_taxid: 3559. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
1.75Å     R-factor:   0.202     R-free:   0.247
Authors: P.Verdino,W.Keller
Key ref: P.Verdino et al. (2008). Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens. J Immunol, 180, 2313-2321. PubMed id: 18250440
Date:
29-Jan-07     Release date:   06-Feb-07    
Supersedes: 1pmz
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q84V36  (POLC3_CHEAL) -  Polcalcin Che a 3
Seq:
Struc:
86 a.a.
81 a.a.
Protein chains
Pfam   ArchSchema ?
Q84V36  (POLC3_CHEAL) -  Polcalcin Che a 3
Seq:
Struc:
86 a.a.
85 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     metal ion binding     2 terms  

 

 
J Immunol 180:2313-2321 (2008)
PubMed id: 18250440  
 
 
Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens.
P.Verdino, R.Barderas, M.Villalba, K.Westritschnig, R.Valenta, R.Rodriguez, W.Keller.
 
  ABSTRACT  
 
Two EF-hand calcium-binding allergens (polcalcins) occur in the pollen of a wide variety of unrelated plants as highly cross-reactive allergenic molecules. We report the expression, purification, immunological characterization, and the 1.75-A crystal structure of recombinant Che a 3 (rChe a 3), the polcalcin from the weed Chenopodium album. The three-dimensional structure of rChe a 3 resembles an alpha-helical fold that is essentially identical with that of the two EF-hand allergens from birch pollen, Bet v 4, and timothy grass pollen, Phl p 7. The extensive cross-reactivity between Che a 3 and Phl p 7 is demonstrated by competition experiments with IgE Abs from allergic patients as well as specific Ab probes. Amino acid residues that are conserved for the two EF-hand allergen family were identified in multiple sequence alignments of polcalcins from 15 different plants. Next, the three-dimensional structures of rChe a 3, rPhl p 7, and rBet v 4 were used to identify conserved amino acids with high surface exposition to visualize surface patches as potential targets for the polyclonal IgE Ab response of allergic patients. The essentially identical three-dimensional structures of rChe a 3, rPhl p 7, and rBet v 4 explain the extensive cross-reactivity of allergic patients IgE Abs with two EF-hand allergens from unrelated plants. In addition, analyzing the three-dimensional structures of cross-reactive Ags for conserved and surface exposed amino acids may be a first approach to mapping the conformational epitopes on disease-related Ags that are recognized by polyclonal patient Abs.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
  20981266 C.H.Schein, O.Ivanciuc, T.Midoro-Horiuti, R.M.Goldblum, and W.Braun (2010).
An Allergen Portrait Gallery: Representative Structures and an Overview of IgE Binding Surfaces.
  Bioinform Biol Insights, 4, 113-125.  
20140203 J.Rouvinen, J.Jänis, M.L.Laukkanen, S.Jylhä, M.Niemi, T.Päivinen, S.Mäkinen-Kiljunen, T.Haahtela, H.Söderlund, and K.Takkinen (2010).
Transient dimers of allergens.
  PLoS One, 5, e9037.  
19111571 J.M.Rolland, L.M.Gardner, and R.E.O'Hehir (2009).
Allergen-related approaches to immunotherapy.
  Pharmacol Ther, 121, 273-284.  
19847289 M.J.Jimenez-Dalmaroni, N.Xiao, A.L.Corper, P.Verdino, G.D.Ainge, D.S.Larsen, G.F.Painter, P.M.Rudd, R.A.Dwek, K.Hoebe, B.Beutler, and I.A.Wilson (2009).
Soluble CD36 ectodomain binds negatively charged diacylglycerol ligands and acts as a co-receptor for TLR2.
  PLoS One, 4, e7411.  
18682111 A.Pomés (2008).
Allergen structures and biologic functions: the cutting edge of allergy research.
  Curr Allergy Asthma Rep, 8, 425-432.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.