PDBsum entry 2o2v

Transferase

PDB id: 2o2v

Contents

Protein chains

92 a.a.

82 a.a.

Waters ×141

PDB id:

2o2v

Name:

Transferase

Title:

Crystal structure of the complex of human mitogen activated protein kinase kinase 5 phox domain (map2k5-phox) with human mitogen activated protein kinase kinase kinase 3 (map3k3b-phox)

Structure:

Dual specificity mitogen-activated protein kinase kinase 5. Chain: a. Fragment: map2k5-phox. Synonym: map kinase kinase 5, mapkk 5, mapk/erk kinase 5. Engineered: yes. Mitogen-activated protein kinase kinase kinase 3. Chain: b. Fragment: map3k3b-phox. Synonym: mapk/erk kinase kinase 3, mek kinase 3, mekk 3.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: map2k5, mek5, mkk5, prkmk5. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: map3k3, mapkkk3, mekk3.

Biol. unit:

Dimer (from PQS)

Resolution:

1.83Å R-factor: 0.196 R-free: 0.224

Authors:

P.Filippakopoulos,P.Savitsky,E.Ugochukwu,A.Edwards,C.Arrowsmith, M.Sundstrom,F.Von Delft,S.Knapp,Structural Genomics Consortium (Sgc)

Key ref:

P.Filippakopoulos et al. Crystal structure of the complex of human mitogen activated protein kinase kinase 5 phox domain (map2k5-Phox) with human mitogen activated protein kinase kinase kinase 3 (map3k3b-Phox). To be published, .

Date:

30-Nov-06 Release date: 12-Dec-06

Protein chain

Q13163  (MP2K5_HUMAN) -  Dual specificity mitogen-activated protein kinase kinase 5 from Homo sapiens

Seq: 448 a.a.

Struc: 92 a.a.

Protein chain

Q99759  (M3K3_HUMAN) -  Mitogen-activated protein kinase kinase kinase 3 from Homo sapiens

Seq: 626 a.a.

Struc: 82 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.7.12.2 - mitogen-activated protein kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
  3. L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺
• Enzyme class 3: Chain B: E.C.2.7.11.25 - mitogen-activated protein kinase kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site