PDBsum entry: 2o0w

Lyase

PDB id

2o0w

Contents

			Protein chain

					399 a.a. *

			Metals

					_CA ×3

			Waters ×638

* Residue conservation analysis

PDB id:

2o0w

Links
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Name:

Lyase

Title:

Pectate lyase bound to hexasaccharide compound iv

Structure:

Pectate lyase. Chain: a. Synonym: pl. Engineered: yes. Mutation: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: pel. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å

R-factor:

0.156

R-free:

0.198

Authors:

R.W.Pickersgill,A.Seyedarabi

Key ref:

A.Seyedarabi et al. Structural and thermodynamic insights into the mechanism of pectate lyase. To be published,

Date:

28-Nov-06

Release date:

20-Nov-07

PROCHECK

	Headers

	References

Protein chain

P39116 (PLY_BACSU) - Pectate lyase

Seq: Struc:					420 a.a. 399 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.4.2.2.2 - Pectate lyase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Pectin and Pectate Lyases

Reaction:

Eliminative cleavage of pectate to give oligosaccharides with 4-deoxy- alpha-D-gluc-4-enuronosyl groups at their non-reducing ends.



		Gene Ontology (GO) functional annotation

Cellular component	extracellular region	1 term
Biochemical function	lyase activity	3 terms