PDBsum entry 2nui

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Hydrolase PDB id
Protein chain
124 a.a. *
_CL ×3
Waters ×155
* Residue conservation analysis
PDB id:
Name: Hydrolase
Title: X-ray structure of synthetic [d83a]rnase a
Structure: Ribonuclease pancreatic. Chain: a. Synonym: rnase 1, rnase a. Engineered: yes. Mutation: yes
Source: Synthetic: yes. Other_details: synthesized using a combination of boc 'in situ neutralization' solid phase peptide synthesis and native chemical ligation. The sequence of the protein is naturally found in bos taurus (bovine).
1.10Å     R-factor:   0.178     R-free:   0.193
Authors: D.J.Boerema,V.A.Tereshko,S.B.H.Kent
Key ref: D.J.Boerema et al. (2008). Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A. Biopolymers, 90, 278-286. PubMed id: 17610259 DOI: 10.1002/bip.20800
09-Nov-06     Release date:   30-Oct-07    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
150 a.a.
124 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     protein binding     5 terms  


DOI no: 10.1002/bip.20800 Biopolymers 90:278-286 (2008)
PubMed id: 17610259  
Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A.
D.J.Boerema, V.A.Tereshko, S.B.Kent.
The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.

Literature references that cite this PDB file's key reference

  PubMed id Reference
  21365769 C.Wang, Q.X.Guo, and Y.Fu (2011).
Theoretical analysis of the detailed mechanism of native chemical ligation reactions.
  Chem Asian J, 6, 1241-1251.  
20593478 C.Li, X.Li, and W.Lu (2010).
Total chemical synthesis of human T-cell leukemia virus type 1 protease via native chemical ligation.
  Biopolymers, 94, 487-494.  
19404746 U.Arnold (2009).
Incorporation of non-natural modules into proteins: structural features beyond the genetic code.
  Biotechnol Lett, 31, 1129-1139.  
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