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PDBsum entry 2nu3

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protein Protein-protein interface(s) links
Hydrolase PDB id
2nu3
Jmol
Contents
Protein chains
185 a.a. *
51 a.a. *
Waters ×151
* Residue conservation analysis
PDB id:
2nu3
Name: Hydrolase
Title: Accommodation of positively-charged residues in a hydrophobic specificity pocket: crystal structures of sgpb in complex with omtky3 variants lys18i and arg18i
Structure: Streptogrisin b, proteinase b. Chain: e. Fragment: residues 115-299. Synonym: protease b, sgpb, pronase enzyme b. Ovomucoid. Chain: i. Fragment: third domain, residues 135-185. Engineered: yes. Mutation: yes
Source: Streptomyces griseus. Organism_taxid: 1911. Strain: k1. Meleagris gallopavo. Turkey. Organism_taxid: 9103. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
1.80Å     R-factor:   0.148    
Authors: K.S.Bateman,S.Anderson,W.Lu,M.A.Qasim,K.Huang,M.Laskowski Jr.,M.N.G.James
Key ref: K.S.Bateman et al. Accommodation of positively-Charged residues in a hydrophobic specificity pocket: crystal structures of sgpb in complex with omtky3 variants lys18i and arg18i. To be published, .
Date:
08-Nov-06     Release date:   21-Nov-06    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00777  (PRTB_STRGR) -  Streptogrisin-B
Seq:
Struc:
299 a.a.
185 a.a.
Protein chain
Pfam   ArchSchema ?
P68390  (IOVO_MELGA) -  Ovomucoid
Seq:
Struc:
185 a.a.
51 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chain E: E.C.3.4.21.81  - Streptogrisin B.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of proteins with specificity similar to trypsin.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     catalytic activity     3 terms