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PDBsum entry 2nn2

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protein Protein-protein interface(s) links
Transcription PDB id
2nn2
Jmol
Contents
Protein chains
117 a.a. *
Waters ×113
* Residue conservation analysis
PDB id:
2nn2
Name: Transcription
Title: Crystal structure of the btb domain from the lrf/zbtb7 transcriptional regulator
Structure: Zinc finger and btb domain-containing protein 7a. Chain: a, b. Fragment: btb/poz domain, residues 1-131. Synonym: leukemia/lymphoma- related factor, factor that binds to inducer of short transcripts protein 1, factor binding ist protein 1, fbi-1, HIV-1 1st-binding protein 1, ttf-i-interacting peptide 21, tip21. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: zbtb7a, fbi1, lrf, zbtb7. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
2.10Å     R-factor:   0.216     R-free:   0.267
Authors: P.J.Stogios,L.Chen,G.G.Prive
Key ref:
P.J.Stogios et al. (2007). Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator. Protein Sci, 16, 336-342. PubMed id: 17189472 DOI: 10.1110/ps.062660907
Date:
23-Oct-06     Release date:   21-Nov-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
O95365  (ZBT7A_HUMAN) -  Zinc finger and BTB domain-containing protein 7A
Seq:
Struc:
 
Seq:
Struc:
584 a.a.
117 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1110/ps.062660907 Protein Sci 16:336-342 (2007)
PubMed id: 17189472  
 
 
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.
P.J.Stogios, L.Chen, G.G.Privé.
 
  ABSTRACT  
 
BTB-zinc finger (BTB-ZF) proteins are transcription regulators with roles in development, differentiation, and oncogenesis. In these proteins, the BTB domain (also known as the POZ domain) is a protein-protein interaction motif that contains a dimerization interface, a possible oligomerization surface, and surfaces for interactions with other factors, including nuclear co-repressors and histone deacetylases. The BTB-ZF protein LRF (also known as ZBTB7, FBI-1, OCZF, and Pokemon) is a master regulator of oncogenesis, and represses the transcription of a variety of important genes, including the ARF, c-fos, and c-myc oncogenes and extracellular matrix genes. We determined the crystal structure of the BTB domain from human LRF to 2.1 A and observed the canonical BTB homodimer fold. However, novel features are apparent on the surface of the homodimer, including differences in the lateral groove and charged pocket regions. The residues that line the lateral groove have little similarity with the equivalent residues from the BCL6 BTB domain, and we show that the 17-residue BCL6 Binding Domain (BBD) from the SMRT co-repressor does not bind to the LRF BTB domain.
 
  Selected figure(s)  
 
 
  The above figure is reprinted by permission from the Protein Society: Protein Sci (2007, 16, 336-342) copyright 2007.  
  Figure was selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
20385364 L.C.Cerchietti, A.F.Ghetu, X.Zhu, G.F.Da Silva, S.Zhong, M.Matthews, K.L.Bunting, J.M.Polo, C.Farès, C.H.Arrowsmith, S.N.Yang, M.Garcia, A.Coop, A.D.Mackerell, G.G.Privé, and A.Melnick (2010).
A small-molecule inhibitor of BCL6 kills DLBCL cells in vitro and in vivo.
  Cancer Cell, 17, 400-411.
PDB code: 3lbz
20133743 T.Ogura, K.I.Tong, K.Mio, Y.Maruyama, H.Kurokawa, C.Sato, and M.Yamamoto (2010).
Keap1 is a forked-stem dimer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains.
  Proc Natl Acad Sci U S A, 107, 2842-2847.  
19170764 N.Ito, M.Watanabe-Matsui, K.Igarashi, and K.Murayama (2009).
Crystal structure of the Bach1 BTB domain and its regulation of homodimerization.
  Genes Cells, 14, 167-178.  
18512963 G.W.Buchko, O.Litvinova, H.Robinson, A.F.Yakunin, and M.A.Kennedy (2008).
Functional and structural characterization of DR_0079 from Deinococcus radiodurans, a novel Nudix hydrolase with a preference for cytosine (deoxy)ribonucleoside 5'-Di- and triphosphates.
  Biochemistry, 47, 6571-6582.
PDB code: 2o5f
  19052359 M.A.Stead, G.O.Rosbrook, J.M.Hadden, C.H.Trinh, S.B.Carr, and S.C.Wright (2008).
Structure of the wild-type human BCL6 POZ domain.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 1101-1104.
PDB code: 3e4u
18368381 M.Laudes, R.Bilkovski, F.Oberhauser, A.Droste, M.Gomolka, U.Leeser, M.Udelhoven, and W.Krone (2008).
Transcription factor FBI-1 acts as a dual regulator in adipogenesis by coordinated regulation of cyclin-A and E2F-4.
  J Mol Med, 86, 597-608.  
18452090 S.Parekh, G.Privé, and A.Melnick (2008).
Therapeutic targeting of the BCL6 oncogene for diffuse large B-cell lymphomas.
  Leuk Lymphoma, 49, 874-882.  
17907153 K.Apostolopoulou, I.S.Pateras, K.Evangelou, P.K.Tsantoulis, M.Liontos, C.Kittas, D.G.Tiniakos, A.Kotsinas, C.Cordon-Cardo, and V.G.Gorgoulis (2007).
Gene amplification is a relatively frequent event leading to ZBTB7A (Pokemon) overexpression in non-small cell lung cancer.
  J Pathol, 213, 294-302.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.