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PDBsum entry 2lmc

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protein Protein-protein interface(s) links
Transcription PDB id
2lmc
Jmol
Contents
Protein chains
59 a.a.
61 a.a.
PDB id:
2lmc
Name: Transcription
Title: Structure of t7 transcription factor gp2-e. Coli rnap jaw do complex
Structure: Bacterial RNA polymerase inhibitor. Chain: a. Engineered: yes. DNA-directed RNA polymerase subunit beta. Chain: b. Fragment: unp residues 1151-1213. Synonym: rnap subunit beta, RNA polymerase subunit beta, transcriptase subunit beta. Engineered: yes
Source: Enterobacteria phage t7. Organism_taxid: 10760. Gene: 2, gp2. Expressed in: escherichia coli. Expression_system_taxid: 562. Escherichia coli k-12. Organism_taxid: 83333. Strain: k12. Gene: b3988, jw3951, rnap, rpoc, tabb.
NMR struc: 10 models
Authors: M.Liu
Key ref: E.James et al. (2012). Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2. Mol Cell, 47, 755-766. PubMed id: 22819324 DOI: 10.1016/j.molcel.2012.06.013
Date:
29-Nov-11     Release date:   14-Mar-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P03704  (VRPI_BPT7) -  Bacterial RNA polymerase inhibitor
Seq:
Struc:
64 a.a.
59 a.a.
Protein chain
Pfam   ArchSchema ?
P0A8T7  (RPOC_ECOLI) -  DNA-directed RNA polymerase subunit beta'
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1407 a.a.
61 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain B: E.C.2.7.7.6  - DNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate
+ RNA(n)
= diphosphate
+ RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     viral reproduction   2 terms 

 

 
    reference    
 
 
DOI no: 10.1016/j.molcel.2012.06.013 Mol Cell 47:755-766 (2012)
PubMed id: 22819324  
 
 
Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2.
E.James, M.Liu, C.Sheppard, V.Mekler, B.Cámara, B.Liu, P.Simpson, E.Cota, K.Severinov, S.Matthews, S.Wigneshweraraj.
 
  ABSTRACT  
 
No abstract given.