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PDBsum entry 2lfj

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protein links
Hydrolase PDB id
2lfj
Jmol
Contents
Protein chain
124 a.a.
PDB id:
2lfj
Name: Hydrolase
Title: Solution structure of the monomeric derivative of bs-rnase
Structure: Seminal ribonuclease. Chain: a. Synonym: s-rnase, seminal rnase, ribonuclease bs-1. Engineered: yes. Mutation: yes
Source: Bos taurus. Cow. Organism_taxid: 9913. Gene: srn. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 10 models
Authors: R.Spadaccini,D.Picone
Key ref: R.Spadaccini et al. (2012). NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping. PLoS One, 7, e29076. PubMed id: 22253705 DOI: 10.1371/journal.pone.0029076
Date:
06-Jul-11     Release date:   08-Feb-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00669  (RNS_BOVIN) -  Seminal ribonuclease
Seq:
Struc:
150 a.a.
124 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     catalytic activity     7 terms  

 

 
DOI no: 10.1371/journal.pone.0029076 PLoS One 7:e29076 (2012)
PubMed id: 22253705  
 
 
NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping.
R.Spadaccini, C.Ercole, M.A.Gentile, D.Sanfelice, R.Boelens, R.Wechselberger, G.Batta, A.Bernini, N.Niccolai, D.Picone.
 
  ABSTRACT  
 
No abstract given.