PDBsum entry 2kq0

Ligase

PDB id: 2kq0

Contents

Protein chain

35 a.a. *

Ligands

THR-ALA-PRO-PRO-GLU-TYR-MET-GLU-ALA

* Residue conservation analysis

PDB id:

2kq0

Name:

Ligase

Title:

Human nedd4 3rd ww domain complex with ebola zaire virus matrix protein vp40 derived peptide ilptappeymea

Structure:

E3 ubiquitin-protein ligase nedd4. Chain: a. Fragment: 3rd ww domain, unp residues 834-878. Synonym: neural precursor cell expressed developmentally down- regulated protein 4, nedd-4, cell proliferation-inducing gene 53 protein. Engineered: yes. 12-mer from matrix protein vp40. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: nedd4. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: bl21(de3). Synthetic: yes. Human t-cell lymphotrophic virus type 1 isolate

NMR struc:

20 models

Authors:

M.Iglesias-Bexiga,M.Macias,R.Bonet,F.J.Blanco,E.S.Cobos,I.Luque

Key ref:

M.Iglesias-Bexiga Human nedd4 3rd ww domain complex with ebola zaire VI matrix protein vp40 derived peptide. To be published, .

Date:

23-Oct-09 Release date: 03-Nov-10

Protein chain

P46934  (NEDD4_HUMAN) -  E3 ubiquitin-protein ligase NEDD4 from Homo sapiens

Seq: 1319 a.a.

Struc: 35 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine