PDBsum entry 2k2t

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protein links
Cell adhesion PDB id
Protein chain
57 a.a.
PDB id:
Name: Cell adhesion
Title: Epidermal growth factor-like domain 2 from toxoplasma gondii microneme protein 6
Structure: Micronemal protein 6. Chain: a. Fragment: egf2-like domain 2 (unp residues 87-147). Engineered: yes
Source: Toxoplasma gondii. Gene: mic6. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 10 models
Authors: K.Sawmynaden,S.Saouros,J.Marchant,P.Simpson,S.Matthews
Key ref: K.Sawmynaden et al. (2008). Structural insights into microneme protein assembly reveal a new mode of EGF domain recognition. EMBO Rep, 9, 1149-1155. PubMed id: 18818666
11-Apr-08     Release date:   24-Feb-09    
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Protein chain
Pfam   ArchSchema ?
Q9XYH7  (MIC6_TOXGO) -  Micronemal protein 6
349 a.a.
57 a.a.
Key:    PfamB domain  Secondary structure  CATH domain


EMBO Rep 9:1149-1155 (2008)
PubMed id: 18818666  
Structural insights into microneme protein assembly reveal a new mode of EGF domain recognition.
K.Sawmynaden, S.Saouros, N.Friedrich, J.Marchant, P.Simpson, B.Bleijlevens, M.J.Blackman, D.Soldati-Favre, S.Matthews.
The obligate intracellular parasite Toxoplasma gondii, a member of the phylum Apicomplexa that includes Plasmodium spp., is one of the most widespread parasites and the causative agent of toxoplasmosis. Adhesive complexes composed of microneme proteins (MICs) are secreted onto the parasite surface from intracellular stores and fulfil crucial roles in host-cell recognition, attachment and penetration. Here, we report the high-resolution solution structure of a complex between two crucial MICs, TgMIC6 and TgMIC1. Furthermore, we identify two analogous interaction sites within separate epidermal growth factor-like (EGF) domains of TgMIC6-EGF2 and EGF3-and confirm that both interactions are functional for the recognition of host cell receptor in the parasite, using immunofluorescence and invasion assays. The nature of this new mode of recognition of the EGF domain and its abundance in apicomplexan surface proteins suggest a more generalized means of constructing functional assemblies by using EGF domains with highly specific receptor-binding properties.

Literature references that cite this PDB file's key reference

  PubMed id Reference
19901027 N.Friedrich, J.M.Santos, Y.Liu, A.S.Palma, E.Leon, S.Saouros, M.Kiso, M.J.Blackman, S.Matthews, T.Feizi, and D.Soldati-Favre (2010).
Members of a novel protein family containing microneme adhesive repeat domains act as sialic acid-binding lectins during host cell invasion by apicomplexan parasites.
  J Biol Chem, 285, 2064-2076.  
20485485 P.W.Chiu, Y.C.Huang, Y.J.Pan, C.H.Wang, and C.H.Sun (2010).
A novel family of cyst proteins with epidermal growth factor repeats in Giardia lamblia.
  PLoS Negl Trop Dis, 4, e677.  
19593815 J.A.Garnett, Y.Liu, E.Leon, S.A.Allman, N.Friedrich, S.Saouros, S.Curry, D.Soldati-Favre, B.G.Davis, T.Feizi, and S.Matthews (2009).
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
  Protein Sci, 18, 1935-1947.
PDB codes: 3f53 3f5a 3f5e
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