PDBsum entry: 2jon

Allergen

PDB id: 2jon

Contents

Protein chain

101 a.a. *

* Residue conservation analysis

PDB id:

2jon

Name:

Allergen

Title:

Solution structure of thE C-terminal domain ole e 9

Structure:

Beta-1,3-glucanase. Chain: a. Fragment: c-terminal domain. Engineered: yes

Source:

Olea europaea. Common olive. Organism_taxid: 4146. Gene: ole9. Expressed in: pichia pastoris. Expression_system_taxid: 4922.

NMR struc:

20 models

Authors:

M.A.Trevino,O.Palomares,I.Castrillo,M.Villalba,R.Rodriguez, M.Rico,J.Santoro,M.Bruix

Key ref:

M.A.Treviño et al. (2008). Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen. Protein Sci, 17, 371-376. PubMed id: 18096638 DOI: 10.1110/ps.073230008

Date:

14-Mar-07 Release date: 29-Jan-08

Protein chain

Q94G86  (Q94G86_OLEEU) -  Beta-1,3-glucanase-like protein

Seq: 460 a.a.

Struc: 101 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1110/ps.073230008

Protein Sci 17:371-376 (2008)

PubMed id: 18096638

Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen.

M.A.Treviño, O.Palomares, I.Castrillo, M.Villalba, R.Rodríguez, M.Rico, J.Santoro, M.Bruix.

ABSTRACT

Ole e 9 is an olive pollen allergen belonging to group 2 of pathogenesis-related proteins. The protein is composed of two immunological independent domains: an N-terminal domain (NtD) with 1,3-beta-glucanase activity, and a C-terminal domain (CtD) that binds 1,3-beta-glucans. We have determined the three-dimensional structure of CtD-Ole e 9 (101 amino acids), which consists of two parallel alpha-helices forming an angle of approximately 55 degrees , a small antiparallel beta-sheet with two short strands, and a 3-10 helix turn, all connected by long coil segments, resembling a novel type of folding among allergens. Two regions surrounded by aromatic residues (F49, Y60, F96, Y91 and Y31, H68, Y65, F78) have been localized on the protein surface, and a role for sugar binding is suggested. The epitope mapping of CtD-Ole e 9 shows that B-cell epitopes are mainly located on loops, although some of them are contained in secondary structural elements. Interestingly, the IgG and IgE epitopes are contiguous or overlapped, rather than coincident. The three-dimensional structure of CtD-Ole e 9 might help to understand the underlying mechanism of its biochemical function and to determine possible structure-allergenicity relationships.

Selected figure(s)

Figure 2.
Figure 2. IgE and IgG epitopes of CtD-Ole e 9. (A) Location of the synthetic peptides used for the epitope mapping, in the CtD-Ole e

The above figure is reprinted by permission from the Protein Society: Protein Sci (2008, 17, 371-376) copyright 2008.