PDBsum entry 2jmc

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protein links
Signaling protein PDB id
Protein chain
77 a.a. *
* Residue conservation analysis
PDB id:
Name: Signaling protein
Title: Chimer between spc-sh3 and p41
Structure: Spectrin alpha chain, brain and p41 peptide chimera. Chain: a. Synonym: spectrin, non-erythroid alpha chain, fodrin alpha chain. Engineered: yes
Source: Gallus gallus. Chicken. Organism_taxid: 9031. Gene: sptan1, spta2. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 20 models
Authors: N.A.J.Van Nuland,A.M.Candel,J.C.Martinez,F.Conejero-Lara, M.Bruix
Key ref: A.M.Candel et al. (2007). The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex. FEBS Lett, 581, 687-692. PubMed id: 17275816 DOI: 10.1016/j.febslet.2007.01.032
02-Nov-06     Release date:   24-Apr-07    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P07751  (SPTA2_CHICK) -  Spectrin alpha chain, non-erythrocytic 1
2477 a.a.
77 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 33 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     endocytosis   1 term 


DOI no: 10.1016/j.febslet.2007.01.032 FEBS Lett 581:687-692 (2007)
PubMed id: 17275816  
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex.
A.M.Candel, F.Conejero-Lara, J.C.Martinez, N.A.van Nuland, M.Bruix.
Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.

Literature references that cite this PDB file's key reference

  PubMed id Reference
20303980 M.Guttman, J.H.Prieto, T.M.Handel, P.J.Domaille, and E.A.Komives (2010).
Structure of the minimal interface between ApoE and LRP.
  J Mol Biol, 398, 306-319.
PDB codes: 2knx 2kny
19617233 A.M.Candel, E.S.Cobos, F.Conejero-Lara, and J.C.Martinez (2009).
Evaluation of folding co-operativity of a chimeric protein based on the molecular recognition between polyproline ligands and SH3 domains.
  Protein Eng Des Sel, 22, 597-606.  
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