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PDBsum entry 2j91

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protein ligands metals Protein-protein interface(s) links
Lyase PDB id
2j91
Jmol
Contents
Protein chain
457 a.a. *
Ligands
AMP ×4
GOL ×4
Metals
_CL ×4
Waters ×1495
* Residue conservation analysis
PDB id:
2j91
Name: Lyase
Title: Crystal structure of human adenylosuccinate lyase in complex with amp
Structure: Adenylosuccinate lyase. Chain: a, b, c, d. Fragment: residues 1-481. Synonym: adenylosuccinase, asl, asase. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Biol. unit: Tetramer (from PDB file)
Resolution:
1.80Å     R-factor:   0.160     R-free:   0.199
Authors: P.Stenmark,M.Moche,C.Arrowsmith,H.Berglund,R.Busam,R.Collins A.Edwards,U.B.Ericsson,S.Flodin,A.Flores,S.Graslund,M.Hamma B.M.Hallberg,L.Holmberg Schiavone,M.Hogbom,I.Johansson,T.Ka U.Kosinska,T.Kotenyova,A.Magnusdottir,M.E.Nilsson,P.Nilsson T.Nyman,D.Ogg,C.Persson,J.Sagemark,M.Sundstrom,J.Uppenberg, M.Uppsten,A.G.Thorsell,S.Van Den Berg,K.Wallden,J.Weigelt, P.Nordlund
Key ref: P.Stenmark et al. Crystal structure of human adenylosuccinate lyase. To be published, .
Date:
01-Nov-06     Release date:   07-Nov-06    
Supersedes: 2j84
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P30566  (PUR8_HUMAN) -  Adenylosuccinate lyase
Seq:
Struc:
484 a.a.
457 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
=
fumarate
Bound ligand (Het Group name = GOL)
matches with 40.00% similarity
+
AMP
Bound ligand (Het Group name = AMP)
corresponds exactly
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
=
fumarate
Bound ligand (Het Group name = GOL)
matches with 40.00% similarity
+
5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Bound ligand (Het Group name = AMP)
matches with 87.50% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     mitochondrion   2 terms 
  Biological process     'de novo' AMP biosynthetic process   17 terms 
  Biochemical function     catalytic activity     4 terms