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PDBsum entry 2j84

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protein ligands metals Protein-protein interface(s) links
Lyase PDB id
2j84
Jmol
Contents
Protein chains
457 a.a.
Ligands
AMP ×4
GOL ×4
Metals
_CL ×4
Waters ×1499
Superseded by: 2j91 2j91
PDB id:
2j84
Name: Lyase
Title: Crystal structure of human adenylosuccinate lyase in complex with amp
Structure: Adenylosuccinate lyase. Synonym: adenylosuccinase, asl, asase. Chain: a, b, c, d. Fragment: residues 1-481. Engineered: yes
Source: Expressed in: escherichia coli. Homo sapiens. Human
Biol. unit: Tetramer (from PDB file)
Resolution:
1.80Å     R-factor:   0.160     R-free:   0.199
Authors: P.Stenmark,M.Moche,C.Arrowsmith,H.Berglund,R.Busam, R.Collins,A.Edwards,U.B.Ericsson,S.Flodin,A.Flores, S.Graslund,M.Hammarstrom,B.M.Hallberg,L.H.Schiavone, M.Hogbom,I.Johansson,T.Karlberg,U.Kosinska,T.Kotenyova, A.Magnusdottir,M.E.Nilsson,P.Nilsson-Ehle,T.Nyman,D.Ogg, C.Persson,J.Sagemark,M.Sundstrom,J.Uppenberg,M.Uppsten, A.G.Thorsell,S.Van Den Berg,K.Wallden,J.Weigelt,P.Nordlund
Key ref: P.Stenmark et al. Crystal structure of human adenylosuccinate lyase. To be published, .
Date:
19-Oct-06     Release date:   23-Oct-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P30566  (PUR8_HUMAN) -  Adenylosuccinate lyase
Seq:
Struc:
484 a.a.
457 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
Bound ligand (Het Group name = AMP)
matches with 74.00% similarity
= fumarate
+ AMP
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
= fumarate
+ 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Molecule diagrams generated from .mol files obtained from the KEGG ftp site