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PDBsum entry 2isi

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protein ligands metals Protein-protein interface(s) links
Lyase PDB id
2isi
Jmol
Contents
Protein chain
276 a.a. *
Ligands
PO4 ×2
Metals
_MG ×3
Waters ×37
* Residue conservation analysis
PDB id:
2isi
Name: Lyase
Title: Crystal structure of ape1 from homo sapiens in a new crystal form complexed with a ligand
Structure: DNA-(apurinic or apyrimidinic site) lyase. Chain: a, b, c. Synonym: ap endonuclease 1, apex nuclease, apen, ref-1 protein. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.76Å     R-factor:   0.228     R-free:   0.284
Authors: R.Agarwal,M.D.Naidu
Key ref: R.Agarwal and m.d.naidu Crystal structure of hape1 in a new crystal form with a bound ligand: implications on catalytic mechanism and its inhibition. To be published, .
Date:
17-Oct-06     Release date:   31-Oct-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P27695  (APEX1_HUMAN) -  DNA-(apurinic or apyrimidinic site) lyase
Seq:
Struc:
318 a.a.
276 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.2.99.18  - DNA-(apurinic or apyrimidinic site) lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   12 terms 
  Biological process     cellular response to cAMP   24 terms 
  Biochemical function     protein binding     27 terms