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Signal protein
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PDB id
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2irt
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Contents |
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* Residue conservation analysis
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* C-alpha coords only
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Gene Ontology (GO) functional annotation
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Cellular component
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extracellular region
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4 terms
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Biological process
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immune response
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3 terms
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Biochemical function
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protein binding
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3 terms
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DOI no:
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Acta Crystallogr D Biol Crystallogr
50:197-201
(1994)
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PubMed id:
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Initial crystallographic analysis of a recombinant human interleukin-1 receptor antagonist protein.
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L.L.Clancy,
B.C.Finzel,
A.W.Yem,
M.R.Deibel,
N.A.Strakalaitis,
D.P.Brunner,
R.M.Sweet,
H.M.Einspahr.
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ABSTRACT
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We report the crystallization of samples of a recombinant preparation of human
interleukin-1 receptor antagonist protein (IRAP) and solution of the crystal
structure by isomorphous replacement methods. Crystals were obtained by the
hanging-drop vapor-diffusion method at 277 K from solutions of PEG 4000
containing sodium chloride, dithiothreitol and PIPES [sodium
buffer at pH 7.0. Crystals appear
within about a week and grow as truncated tetragonal bipyramids to 0.3-0.6 mm on
an edge. X-ray diffraction data from these crystals specify space group
P4(3)2(1)2 and unit-cell dimensions of a = b = 72.35(26), c = 114.7(8) A and Z =
16 (two molecules per asymmetric unit). Fresh crystals diffract to about 2.3 A
resolution. The search for heavy-atom derivatives has produced two, potassium
gold cyanide and trimethyl lead chloride, as same-site, single-site derivatives.
Inspection of an electron-density map at 4 A resolution calculated with these
derivatives confirms that the IRAP molecule is a member of the interleukin-1
structural family.
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Selected figure(s)
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Figure 3.
Fig. 3. Schematic illustrations of the molecular structures of
IL-lfl, IL-la and I RAP. Each illustration traces the course of a
polypeptide chain, with /3-strand positions and directions
indicated with broad arrows. The left view is down the axis of
the six-stranded fl-barrel (shaded darker and in front). In the
right view the barrel axis is rotated to lie in the plane of the
page. These illustrations were made with the
MOLSCRIPT
programs (Kraulis, 1991).
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The above figure is
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1994,
50,
197-201)
copyright 1994.
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Figure was
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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H.A.Schreuder,
J.M.Rondeau,
C.Tardif,
A.Soffientini,
E.Sarubbi,
A.Akeson,
T.L.Bowlin,
S.Yanofsky,
and
R.W.Barrett
(1995).
Refined crystal structure of the interleukin-1 receptor antagonist. Presence of a disulfide link and a cis-proline.
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Eur J Biochem, 227,
838-847.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
