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Antibiotic/antibiotic inhibitor PDB-id
2gzj
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Contents
Description
Header details
Header records
References
PROCHECK
Protein chains
83 a.a. *
130 a.a. *
Ligands
PO4 ×2
Metal ions
_ZN ×2
Waters ×686

* Residue conservation analysis
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PDB id: 2gzj
Name: Antibiotic/antibiotic inhibitor
Title: Crystal structure of the e9 dnase domain with a mutant immunity protein im9 (d51a)

Structure:
Colicin-e9 immunity protein. Chain: a, e. Synonym: imme9, microcin-e9 immunity protein. Engineered: yes. Mutation: yes. Colicin-e9. Chain: b, f. Fragment: colicin e9,c-terminal domain,dnase domain. Engineered: yes

Source:
Escherichia coli k12. Organism_taxid: 83333. Strain: k-12. Gene: imm, ceie9. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: col, cei. Expressed in: escherichia coli. Expression_system_taxid: 562.

UniProt:
Chains A, E: P13479 (IMM9_ECOLX)
Pfam   ArchSchema ?
Seq: 86 a.a.
Struc: 83 a.a.*

Chains B, F: P09883 (CEA9_ECOLX)
Pfam   ArchSchema ?
Seq:
Struc:
Seq:
Struc:
Seq: 582 a.a.
Struc: 130 a.a.
Key:    PfamA domain
 Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

Resolution:
1.60Å

R-factor:
0.187

R-free:
0.232

Authors:
P.S.Santi,O.O.Kolade,U.C.Kuhlmann,C.Kleanthous,A.M.Hemmings

Key ref:
u.c.kuhlmann et al. To be Published, .

Date:
11-May-06

Release date:
18-Mar-08

Related entries:
Iemv
crystal structure of colicin e9 dnase domain with its
cognate immunity protein im9 (1.7 angstroms)
1bxi
crystal structure of the escherichia coli colicin e9 dnase
domain with its cognate immunity protein im9
2gyk
crystal structures of the complexes of the colicin e9 dnase
domain with mutant immunity proteins
2gze
... plus others (see Header records)
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