PDBsum entry: 2gyk

Antibiotic/antibiotic inhibitor

PDB id: 2gyk

Contents

Protein chains

83 a.a. *

130 a.a. *

Ligands

PO4 ×2

Metals

_ZN ×2

Waters ×686

* Residue conservation analysis

PDB id:

2gyk

Name:

Antibiotic/antibiotic inhibitor

Title:

Crystal structure of the complex of the colicin e9 dnase dom mutant immunity protein, imme9 (d51a)

Structure:

Colicin-e9 immunity protein. Chain: a, e. Synonym: imme9, microcin-e9 immunity protein. Engineered: yes. Mutation: yes. Colicin-e9. Chain: b, f. Fragment: c-terminal domain, dnase domain. Engineered: yes

Source:

Escherichia coli k12. Organism_taxid: 83333. Strain: k-12. Gene: imm9_ecoli. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: col, cei. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.187 R-free: 0.232

Authors:

P.S.Santi,O.O.Kolade,U.C.Kuhlmann,A.M.Hemmings

Key ref:

U.C.Kuhlmann et al. Crystal structures of the complexes of the colicin e9 dnase domain with mutant immunity proteins. To be published,

Date:

09-May-06 Release date: 15-May-07

Protein chains

P13479  (IMM9_ECOLX) -  Colicin-E9 immunity protein

Seq: 86 a.a.

Struc: 83 a.a.*

Protein chains

P09883  (CEA9_ECOLX) -  Colicin-E9

Seq: 582 a.a.

Struc: 130 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 

• Biological process: cytolysis (4 terms)
• Biochemical function: protein binding (4 terms)