PDBsum entry: 2gui

Transferase

PDB id

2gui

Contents

			Protein chain

					176 a.a. *

			Ligands

					U5P ×2


					EDO ×2


					PEG

			Metals

					_MN ×2

			Waters ×240

* Residue conservation analysis

PDB id:

2gui

Links
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Name:

Transferase

Title:

Structure and function of cyclized versions of the proofread exonuclease subunit of e. Coli DNA polymerase iii

Structure:

DNA polymerase iii epsilon subunit. Chain: a. Fragment: n-terminal exonuclease domain, residues 2-186. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: dnaq, mutd. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å

R-factor:

0.162

R-free:

0.197

Authors:

A.Y.Park,P.D.Carr,D.L.Ollis,N.E.Dixon

Key ref:

A.Y.Park et al. Structure and function of cyclized versions of the proofreading exonuclease subunit e. Coli DNA polymera. To be published,

Date:

30-Apr-06

Release date:

13-Mar-07

PROCHECK

	Headers

	References

Protein chain

P03007 (DPO3E_ECOLI) - DNA polymerase III subunit epsilon

Seq: Struc:					243 a.a. 176 a.a.

Key:

PfamA domain

PfamB domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.2.7.7.7 - DNA-directed Dna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Deoxynucleoside triphosphate
Bound ligand (Het Group name = U5P)
matches with 41.00% similarity

DNA(n)

diphosphate

DNA(n+1)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site



		Gene Ontology (GO) functional annotation

Cellular component	intracellular	1 term
Biological process	DNA replication	1 term
Biochemical function	nucleic acid binding	4 terms