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Transcription PDB id
2grl
Jmol
Contents
Protein chains
287 a.a. *
Ligands
ALA-ILE-THR-LEU-
ILE-PHE-ILE
Waters ×46
* Residue conservation analysis
PDB id:
2grl
Name: Transcription
Title: Crystal structure of dct/icf10 complex
Structure: Prgx. Chain: a, b, c, d. Engineered: yes. Peptide. Chain: e. Engineered: yes
Source: Enterococcus faecalis. Organism_taxid: 1351. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Resolution:
3.00Å     R-factor:   0.238     R-free:   0.271
Authors: K.Shi,B.K.Kozlowicz,Z.Y.Gu,D.H.Ohlendorf,C.A.Earhart, G.M.Dunny
Key ref: B.K.Kozlowicz et al. (2006). Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Mol Microbiol, 62, 958-969. PubMed id: 17038121 DOI: 10.1111/j.1365-2958.2006.05434.x
Date:
24-Apr-06     Release date:   03-Apr-07    
PROCHECK
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 Headers
 References

 

 
DOI no: 10.1111/j.1365-2958.2006.05434.x Mol Microbiol 62:958-969 (2006)
PubMed id: 17038121  
 
 
Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.
B.K.Kozlowicz, K.Shi, Z.Y.Gu, D.H.Ohlendorf, C.A.Earhart, G.M.Dunny.
 
  ABSTRACT  
 
In many bacteria expression of lateral gene transfer and of virulence factors is controlled by cell-cell signalling systems. Molecular interactions of microbial signal molecules with their cognate receptors are not well understood. For the Enterococcus faecalis conjugative plasmid pCF10, the PrgX protein serves as a molecular switch controlling expression of conjugation and virulence genes encoded by the plasmid. The induction state of a pCF10-carrying donor cell is determined by the ratio of two signalling peptides, cCF10 pheromone and iCF10 inhibitor. Recent analysis of PrgX/cCF10 interactions suggests a mechanism for conversion to the induced state. However, the means by which iCF10 peptide antagonizes cCF10 activity is unclear, and it has been suggested that inhibitor peptides block import of pheromone peptides. We now show that both of these peptides interact with the same binding pocket of PrgX, but they differentially alter the conformation of the protein and its oligomerization state, resulting in opposing biological activities.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20332804 D.J.Rankin, E.P.Rocha, and S.P.Brown (2011).
What traits are carried on mobile genetic elements, and why?
  Heredity, 106, 1.  
21353627 G.M.Dunny, and C.M.Johnson (2011).
Regulatory circuits controlling enterococcal conjugation: lessons for functional genomics.
  Curr Opin Microbiol, 14, 174-180.  
20097859 C.M.Johnson, D.A.Manias, H.A.Haemig, S.Shokeen, K.E.Weaver, T.M.Henkin, and G.M.Dunny (2010).
Direct evidence for control of the pheromone-inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript-driven attenuation mechanism.
  J Bacteriol, 192, 1634-1642.  
  20827374 R.J.Bennett, and G.M.Dunny (2010).
Analogous telesensing pathways regulate mating and virulence in two opportunistic human pathogens.
  MBio, 1, 0.  
19805280 G.Zhao, G.Li, H.Schindelin, and W.J.Lennarz (2009).
An Armadillo motif in Ufd3 interacts with Cdc48 and is involved in ubiquitin homeostasis and protein degradation.
  Proc Natl Acad Sci U S A, 106, 16197-16202.
PDB code: 3gae
18083822 J.R.Chandler, and G.M.Dunny (2008).
Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgY.
  J Bacteriol, 190, 1172-1183.  
17360276 G.M.Dunny (2007).
The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolution.
  Philos Trans R Soc Lond B Biol Sci, 362, 1185-1193.  
17098891 K.R.Fixen, J.R.Chandler, T.Le, B.K.Kozlowicz, D.A.Manias, and G.M.Dunny (2007).
Analysis of the amino acid sequence specificity determinants of the enterococcal cCF10 sex pheromone in interactions with the pheromone-sensing machinery.
  J Bacteriol, 189, 1399-1406.  
17921293 M.Ibrahim, A.Guillot, F.Wessner, F.Algaron, C.Besset, P.Courtin, R.Gardan, and V.Monnet (2007).
Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system?
  J Bacteriol, 189, 8844-8854.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.