PDBsum entry: 2fna

Atp-binding protein

PDB id: 2fna

Contents

Protein chains

352 a.a. *

Ligands

ADP ×2

EDO ×7

Metals

_MG ×2

Waters ×348

* Residue conservation analysis

PDB id:

2fna

Name:

Atp-binding protein

Title:

Crystal structure of an archaeal aaa+ atpase (sso1545) from solfataricus p2 at 2.00 a resolution

Structure:

Conserved hypothetical protein. Chain: a, b. Engineered: yes

Source:

Sulfolobus solfataricus. Organism_taxid: 273057. Strain: p2. Gene: 13814777. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å R-factor: 0.177 R-free: 0.226

Authors:

Joint Center For Structural Genomics (Jcsg)

Key ref:

Q.Xu et al. (2009). Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus. Proteins, 74, 1041-1049. PubMed id: 19089981 DOI: 10.1002/prot.22325

Date:

10-Jan-06 Release date: 07-Feb-06

Protein chains

Q97Y08  (Q97Y08_SULSO) -  Putative uncharacterized protein

Seq: 356 a.a.

Struc: 352 a.a.

 Gene Ontology (GO) functional annotation 

• Biochemical function: nucleotide binding (2 terms)

DOI no: 10.1002/prot.22325

Proteins 74:1041-1049 (2009)

PubMed id: 19089981

Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus.

Q.Xu, C.L.Rife, D.Carlton, M.D.Miller, S.S.Krishna, M.A.Elsliger, P.Abdubek, T.Astakhova, H.J.Chiu, T.Clayton, L.Duan, J.Feuerhelm, S.K.Grzechnik, J.Hale, G.W.Han, L.Jaroszewski, K.K.Jin, H.E.Klock, M.W.Knuth, A.Kumar, D.McMullan, A.T.Morse, E.Nigoghossian, L.Okach, S.Oommachen, J.Paulsen, R.Reyes, H.van den Bedem, K.O.Hodgson, J.Wooley, A.M.Deacon, A.Godzik, S.A.Lesley, I.A.Wilson.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 2.
Figure 2. Surface conservation pattern and the nucleotide binding site of SSO1545. A: Molecular surface of SSO1545 colored by sequence conservation. The most conserved residues are shown in red, the nonconserved residues in white. The three domains of SSO1543 are shown in ribbon representation and colored as yellow, blue, and cyan, respectively. The orientation of left panel is the same as in Figure 1(A). B: Close-up stereo view of the ADP binding site. The bound ADP (yellow) and magnesium ion (silver) are shown in sticks and sphere, respectively. Walker A (P-loop, green), Walker B (W-B, blue), sensor I (S-I, cyan), sensor II (S-II, white) are shown in cartoon and sticks. The STAND-specific hhGRExE and GxP motifs are also highlighted in blue.

Figure 3.
Figure 3. Structural comparisons of SSO1545 with AAA+ ATPases with similar domain organizations: Orc1 (PDB 2v1u), RuvB (PDB 1in5), Orc2 (PDB 1w5t), and Apaf-1 (PDB 1z6t, residues 105-450). These structures were superimposed base on their respective NTPase domains (gray). The linker regions connecting the NTPase domain and the WH domain (green) are shown in red. The nucleotides and magnesium ions are shown in sticks and spheres, respectively.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2009, 74, 1041-1049) copyright 2009.

Figures were selected by an automated process.