 |
PDBsum entry 2fkd
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Transcription regulator
|
PDB id
|
|
|
|
2fkd
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Transcription regulator
|
|
|
Title:
|
|
Crystal structure of thE C-terminal domain of bacteriophage 186 repressor
|
|
Structure:
|
|
Repressor protein ci. Chain: a, b, c, d, e, f, g, h, i, j, k, l, m, n. Fragment: c-terminal domain. Engineered: yes
|
|
Source:
|
|
Enterobacteria phage 186. Organism_taxid: 29252. Gene: ci. Expressed in: escherichia coli. Expression_system_taxid: 562
|
|
Biol. unit:
|
|
40mer (from
)
|
|
Resolution:
|
|
|
2.70Å
|
R-factor:
|
0.240
|
R-free:
|
0.297
|
|
|
Authors:
|
|
M.Lewis
|
Key ref:
|
|
H.W.Pinkett
et al.
(2006).
The structural basis of cooperative regulation at an alternate genetic switch.
Mol Cell,
21,
605-615.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
04-Jan-06
|
Release date:
|
13-Jun-06
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P08707
(RPC1_BP186) -
Repressor protein CI from Escherichia phage 186
|
|
|
|
Seq:
Struc:
|
|
|
|
192 a.a.
110 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Mol Cell
21:605-615
(2006)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
The structural basis of cooperative regulation at an alternate genetic switch.
|
|
H.W.Pinkett,
K.E.Shearwin,
S.Stayrook,
I.B.Dodd,
T.Burr,
A.Hochschild,
J.B.Egan,
M.Lewis.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
Bacteriophage lambda is a paradigm for understanding the role of cooperativity
in gene regulation. Comparison of the regulatory regions of lambda and the
unrelated temperate bacteriophage 186 provides insight into alternate ways to
assemble functional genetic switches. The structure of the C-terminal domain of
the 186 repressor, determined at 2.7 A resolution, reveals an unusual heptamer
of dimers, consistent with presented genetic studies. In addition, the structure
of a cooperativity mutant of the full-length 186 repressor, identified by
genetic screens, was solved to 1.95 A resolution. These structures provide a
molecular basis for understanding lysogenic regulation in 186. Whereas the
overall fold of the 186 and lambda repressor monomers is remarkably similar, the
way the two repressors cooperatively assemble is quite different and explains in
part the differences in their regulatory activity.
|
|
|
|
|
|
| |
Selected figure(s)
|
|
|
|
| |
|
|
|
|
|
|
Figure 1.
Figure 1. Comparison of the λ and 186 Switch Regions
|
|
Figure 2.
Figure 2. Structures of the CTD of λ and 186 Repressor
|
|
|
|
|
|
| |
The above figures are
reprinted
by permission from Cell Press:
Mol Cell
(2006,
21,
605-615)
copyright 2006.
|
|
| |
Figures were
selected
by an automated process.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
N.Hao,
M.L.Whitelaw,
K.E.Shearwin,
I.B.Dodd,
and
A.Chapman-Smith
(2011).
Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR.
|
| |
Nucleic Acids Res,
39,
3695-3709.
|
|
|
|
|
|
|
M.Pedersen,
M.Ligowska,
and
K.Hammer
(2010).
Characterization of the CI repressor protein encoded by the temperate lactococcal phage TP901-1.
|
| |
J Bacteriol,
192,
2102-2110.
|
|
|
|
|
|
|
T.Massad,
K.Skaar,
H.Nilsson,
P.Damberg,
P.Henriksson-Peltola,
E.Haggård-Ljungquist,
M.Högbom,
and
P.Stenmark
(2010).
Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.
|
| |
Nucleic Acids Res,
38,
7778-7790.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
A.Ahlgren-Berg,
P.Henriksson-Peltola,
W.Sehlén,
and
E.Haggård-Ljungquist
(2007).
A comparison of the DNA binding and bending capacities and the oligomeric states of the immunity repressors of heteroimmune coliphages P2 and WPhi.
|
| |
Nucleic Acids Res,
35,
3167-3180.
|
|
|
|
|
|
|
P.Henriksson-Peltola,
W.Sehlén,
and
E.Haggård-Ljungquist
(2007).
Determination of the DNA-binding kinetics of three related but heteroimmune bacteriophage repressors using EMSA and SPR analysis.
|
| |
Nucleic Acids Res,
35,
3181-3191.
|
|
|
|
|
|
|
D.Ndjonka,
and
C.E.Bell
(2006).
Structure of a hyper-cleavable monomeric fragment of phage lambda repressor containing the cleavage site region.
|
| |
J Mol Biol,
362,
479-489.
|
|
|
PDB codes:
|
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
|
Links
