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PDBsum entry 2ff0

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protein dna_rna metals links
Hormone/growth factor/DNA PDB id
2ff0
Jmol
Contents
Protein chain
102 a.a. *
DNA/RNA
Metals
_ZN ×2
* Residue conservation analysis
PDB id:
2ff0
Name: Hormone/growth factor/DNA
Title: Solution structure of steroidogenic factor 1 DNA binding domain bound to its target sequence in the inhibin alpha- subunit promoter
Structure: Steroidogenic factor 1. Chain: a. Fragment: DNA binding domain. Synonym: stf-1, sf-1, adrenal 4 binding protein, steroid hormone receptor ad4bp, fushi tarazu factor homolog 1, embryonal ltr binding protein, elp, embryonal long terminal repeat- binding protein, steroid hydroxylase positive regulator. Engineered: yes.
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: nr5a1, ftzf1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Other_details: the DNA sequence was synthesized via automated methods.
NMR struc: 16 models
Authors: T.H.Little,Y.Zhang,C.K.Matulis,J.Weck,Z.Zhang, A.Ramachandran,K.E.Mayo,I.Radhakrishnan
Key ref: T.H.Little et al. (2006). Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability. Mol Endocrinol, 20, 831-843. PubMed id: 16339274 DOI: 10.1210/me.2005-0384
Date:
17-Dec-05     Release date:   11-Apr-06    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P33242  (STF1_MOUSE) -  Steroidogenic factor 1
Seq:
Struc:
462 a.a.
102 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     nucleus   1 term 
  Biological process     regulation of transcription, DNA-dependent   1 term 
  Biochemical function     DNA binding     6 terms  

 

 
DOI no: 10.1210/me.2005-0384 Mol Endocrinol 20:831-843 (2006)
PubMed id: 16339274  
 
 
Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability.
T.H.Little, Y.Zhang, C.K.Matulis, J.Weck, Z.Zhang, A.Ramachandran, K.E.Mayo, I.Radhakrishnan.
 
  ABSTRACT  
 
Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear hormone receptors and is considered a master regulator of reproduction because it regulates a number of genes encoding reproductive hormones and enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-alpha gene that encodes a subunit of a reproductive hormone. SF1 forms a specific complex with the DNA through a bipartite motif binding to the major and minor grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric receptors, nerve growth factor-induced-B and estrogen-related receptor 2. However, unlike these receptors, SF1 harbors a helix in the C-terminal segment of the FTZ-F1 box that interacts with both the core DBD and DNA and serves as an important determinant of stability of the complex. We propose that the FTZ-F1 helix along with the core DBD serves as a platform for interactions with coactivators and other DNA-bound factors in the vicinity.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19188450 B.Xu, W.H.Yang, I.Gerin, C.D.Hu, G.D.Hammer, and R.J.Koenig (2009).
Dax-1 and steroid receptor RNA activator (SRA) function as transcriptional coactivators for steroidogenic factor 1 in steroidogenesis.
  Mol Cell Biol, 29, 1719-1734.  
19863811 S.A.Thomson, W.S.Baldwin, Y.H.Wang, G.Kwon, and G.A.Leblanc (2009).
Annotation, phylogenetics, and expression of the nuclear receptors in Daphnia pulex.
  BMC Genomics, 10, 500.  
18838537 L.A.Campbell, E.J.Faivre, M.D.Show, J.G.Ingraham, J.Flinders, J.D.Gross, and H.A.Ingraham (2008).
Decreased recognition of SUMO-sensitive target genes following modification of SF-1 (NR5A1).
  Mol Cell Biol, 28, 7476-7486.  
  18987494 L.Lin, and J.C.Achermann (2008).
Steroidogenic factor-1 (SF-1, Ad4BP, NR5A1) and disorders of testis development.
  Sex Dev, 2, 200-209.  
18651760 M.D.Krasowski, E.J.Reschly, and S.Ekins (2008).
Intrinsic disorder in nuclear hormone receptors.
  J Proteome Res, 7, 4359-4372.  
17200175 L.Lin, P.Philibert, B.Ferraz-de-Souza, D.Kelberman, T.Homfray, A.Albanese, V.Molini, N.J.Sebire, S.Einaudi, G.S.Conway, I.A.Hughes, J.L.Jameson, C.Sultan, M.T.Dattani, and J.C.Achermann (2007).
Heterozygous missense mutations in steroidogenic factor 1 (SF1/Ad4BP, NR5A1) are associated with 46,XY disorders of sex development with normal adrenal function.
  J Clin Endocrinol Metab, 92, 991-999.  
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