PDBsum entry 2fbb

Hydrolase

PDB id: 2fbb

Contents

Protein chain

129 a.a. *

Ligands

NO3 ×16

Metals

_NA ×5

Waters ×165

* Residue conservation analysis

PDB id:

2fbb

Name:

Hydrolase

Title:

Crystal structure analysis of hexagonal lysozyme

Structure:

LysozymE C. Chain: a. Synonym: 1,4-beta-n-acetylmuramidasE C, allergen gal d 4, gal d iv. Ec: 3.2.1.17

Source:

Gallus gallus. Chicken. Organism_taxid: 9031

Resolution:

1.46Å R-factor: 0.183 R-free: 0.198

Authors:

C.Brinkmann,M.S.Weiss,E.Weckert

Key ref:

C.Brinkmann et al. (2006). The structure of the hexagonal crystal form of hen egg-white lysozyme. Acta Crystallogr D Biol Crystallogr, 62, 349-355. PubMed id: 16552135 DOI: 10.1107/S0907444906000825

Date:

09-Dec-05 Release date: 28-Mar-06

Protein chain

P00698  (LYSC_CHICK) -  Lysozyme C from Gallus gallus

Seq: 147 a.a.

Struc: 129 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

DOI no: 10.1107/S0907444906000825

Acta Crystallogr D Biol Crystallogr 62:349-355 (2006)

PubMed id: 16552135

The structure of the hexagonal crystal form of hen egg-white lysozyme.

C.Brinkmann, M.S.Weiss, E.Weckert.

ABSTRACT

The three-dimensional structure of hen egg-white lysozyme (HEWL) in a hexagonal crystal form has been determined and refined to 1.46 A resolution. This hexagonal crystal form crystallizes from a saturated sodium nitrate solution at pH 8.4. The crystals belong to space group P6(1)22, with unit-cell parameters a = b = 85.64, c = 67.93 A. A total of 165 water molecules, 16 nitrate ions and five sodium ions were located in the electron-density map. The hexagonal crystal form exhibits a higher solvent content and a higher degree of disorder than other crystal forms of lysozyme. The flexibility of the protein depends on the crystal packing, although some residue ranges are flexible in all native HEWL crystal forms.

Selected figure(s)

Figure 2.
Figure 2 (a) and (b) Na132 and (c) and (d) Na133 in the hexagonal HEWL crystal form. The twofold symmetry axes are indicated. The distances between sodium and connected O atoms are shown in (b) and (d), respectively. (2F[o] - F[c]) maps contoured at 1.0 . Distances are given in Å. Figures were generated using MOLSCRIPT v2.1.2 (Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24, 946-950.]), BOBSCRIPT (Esnouf, 1997[Esnouf, R. M. (1997). J. Mol. Graph. 15, 132-134.]) and RASTER3D v2.7b (Merritt & Bacon, 1997[Merritt, E. A. & Bacon, D. J. (1997). Methods Enzymol. 277, 505-524.]).

Figure 3.
Figure 3 C^ superposition of HEWL crystal forms (triclinic, monoclinic, orthorhombic and tetragonal structures; Brinkmann et al., 2006[Brinkmann, C., Weiss, M. S. & Weckert, E. (2006). In preparation.]) and of HEWL, GEWL and TEWL. Calculations were performed using the program LSQKAB (Collaborative Computional Project, Number 4, 1994[Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.]). Figures were generated using MOLSCRIPT v2.1.2 (Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24, 946-950.]).

The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2006, 62, 349-355) copyright 2006.

Figures were selected by the author.