PDBsum entry 2fa2

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Transferase PDB id
Protein chains
333 a.a. *
SCN ×6
Waters ×27
* Residue conservation analysis
PDB id:
Name: Transferase
Title: Crystal structure of fus3 without a peptide from ste5
Structure: Mitogen-activated protein kinase fus3. Chain: a, b. Synonym: map kinase fus3. Engineered: yes. Mutation: yes
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: fus3, dac2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
2.85Å     R-factor:   0.209     R-free:   0.285
Authors: R.P.Bhattacharyya,A.Remenyi,M.C.Good,C.J.Bashor,A.M.Falick, W.A.Lim
Key ref:
A.Breitkreutz and M.Tyers (2006). Cell signaling. A sophisticated scaffold wields a new trick. Science, 311, 789-790. PubMed id: 16469909 DOI: 10.1126/science.1124620
06-Dec-05     Release date:   28-Mar-06    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P16892  (FUS3_YEAST) -  Mitogen-activated protein kinase FUS3
353 a.a.
333 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.  - Mitogen-activated protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
+ protein
+ phosphoprotein
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     periplasmic space   4 terms 
  Biological process     conjugation   13 terms 
  Biochemical function     nucleotide binding     10 terms  


DOI no: 10.1126/science.1124620 Science 311:789-790 (2006)
PubMed id: 16469909  
Cell signaling. A sophisticated scaffold wields a new trick.
A.Breitkreutz, M.Tyers.
No abstract given.

  Selected figure(s)  
Figure 1.
The mating pathway in budding yeast. In response to ligand-induced activation of a pheromone receptor, activated G protein subunits in the membrane recruit and oligomerize Ste5, which in conjunction with the PAK-like kinase Ste20, activates the MAPK module. Once activated, the MAPKs Fus3 and Kss1 phosphorylate a variety of proteins that effect the pheromone response, including the transcription factor Ste12 and the polarization factor and cyclin-dependent kinase inhibitor Far1. Not all signaling components or substrates are shown [see (2) for details]. (Inset) Circle indicates peptide motif from Ste5 that docks specifically into the A site of Fus3. Squares indicate the peptide motifs from Ste5, the MAPK kinase Ste7, and various substrates that dock into the B sites of both Fus3 and Kss1; the star on Far1 indicates that it is a Fus3-specific substrate (9).
  The above figure is reprinted by permission from the AAAs: Science (2006, 311, 789-790) copyright 2006.