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PDBsum entry 2f8v

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protein ligands Protein-protein interface(s) links
Contractile protein/contractile protein PDB id
2f8v
Jmol
Contents
Protein chains
194 a.a. *
88 a.a. *
Ligands
SO4 ×5
Waters ×79
* Residue conservation analysis
PDB id:
2f8v
Name: Contractile protein/contractile protein
Title: Structure of full length telethonin in complex with the n-te titin
Structure: N2b-titin isoform. Chain: a, b, c, d. Fragment: domains z1z2, residues 1-196. Engineered: yes. Telethonin. Chain: t, y. Synonym: titin cap protein. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: tcap. Expression_system_taxid: 562
Biol. unit: Trimer (from PQS)
Resolution:
2.75Å     R-factor:   0.267     R-free:   0.287
Authors: N.Pinotsis,M.Petoukhov,S.Lange,D.Svergun,P.Zou,M.Gautel,M.Wi
Key ref: N.Pinotsis et al. (2006). Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus. J Struct Biol, 155, 239-250. PubMed id: 16713295 DOI: 10.1016/j.jsb.2006.03.028
Date:
04-Dec-05     Release date:   27-Jun-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q8WZ42  (TITIN_HUMAN) -  Titin
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34350 a.a.
194 a.a.
Protein chains
Pfam   ArchSchema ?
O15273  (TELT_HUMAN) -  Telethonin
Seq:
Struc:
167 a.a.
88 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D: E.C.2.7.11.1  - Non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
= ADP
+ phosphoprotein
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   5 terms 
  Biological process     cardiac muscle hypertrophy   19 terms 
  Biochemical function     BMP binding     8 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.jsb.2006.03.028 J Struct Biol 155:239-250 (2006)
PubMed id: 16713295  
 
 
Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus.
N.Pinotsis, M.Petoukhov, S.Lange, D.Svergun, P.Zou, M.Gautel, M.Wilmanns.
 
  ABSTRACT  
 
The Z-disk region defines the lateral boundary of the sarcomere and requires a high level of mechanical strength to provide a stable framework for large filamentous muscle proteins. The level of complexity at this area is reflected by a large number of protein-protein interactions. Recently, we unraveled how the N-terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel (2:1) sandwich complex by the N-terminal titin-binding segment of the Z-disk ligand telethonin/T-cap [Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M., Wilmanns, M., 2006. Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Nature 439, 229-233]. In this contribution, we present structural data of a related complex of the titin N-terminus with full-length telethonin. The C-terminus of telethonin remains invisible, suggesting that it does not fold into a defined structure even in the presence of titin. In contrast to the structure with truncated telethonin, a dimer of two titin/telethonin complexes is formed within the crystal environment, potentially indicating the formation of higher oligomers. We further investigated the structure and dynamics of this assembly by small-angle X-ray scattering, circular dichroism, and in vivo complementation data. The data consistently indicate the involvement of the C-terminal part of telethonin into the assembly of two titin/telethonin complexes.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19488834 H.Cheng, X.Xu, S.Zhao, B.Liu, M.Yu, and B.Fan (2010).
Molecular cloning and expression profile analysis of porcine TCAP gene.
  Mol Biol Rep, 37, 1641-1647.  
20235223 T.Sadikot, C.R.Hammond, and M.B.Ferrari (2010).
Distinct roles for telethonin N-versus C-terminus in sarcomere assembly and maintenance.
  Dev Dyn, 239, 1124-1135.  
  19789381 A.Kontrogianni-Konstantopoulos, M.A.Ackermann, A.L.Bowman, S.V.Yap, and R.J.Bloch (2009).
Muscle giants: molecular scaffolds in sarcomerogenesis.
  Physiol Rev, 89, 1217-1267.  
18579686 A.L.Bowman, D.H.Catino, J.C.Strong, W.R.Randall, A.Kontrogianni-Konstantopoulos, and R.J.Bloch (2008).
The rho-guanine nucleotide exchange factor domain of obscurin regulates assembly of titin at the Z-disk through interactions with Ran binding protein 9.
  Mol Biol Cell, 19, 3782-3792.  
18389081 I.Bunikis, K.Denker, Y.Ostberg, C.Andersen, R.Benz, and S.Bergström (2008).
An RND-type efflux system in Borrelia burgdorferi is involved in virulence and resistance to antimicrobial compounds.
  PLoS Pathog, 4, e1000009.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.