PDBsum entry: 2f7q

Hydrolase

PDB id: 2f7q

Contents

Protein chain

1014 a.a. *

Ligands

NAG

AOL

MPD

Metals

_ZN

Waters ×1145

* Residue conservation analysis

PDB id:

2f7q

Name:

Hydrolase

Title:

Golgi alpha-mannosidase ii complex with aminocyclopentitetro

Structure:

Alpha-mannosidase ii. Chain: a. Fragment: catalytic domain. Synonym: mannosyl-oligosaccharide 1,3-1,6- alpha-mannosidas golgi alpha-mannosidase ii. Aman ii. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: alpha-man-ii, gmii. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227.

Biol. unit:

Monomer (from PQS)

Resolution:

1.85Å R-factor: 0.150 R-free: 0.190

Authors:

D.A.Kuntz,D.R.Rose

Key ref:

S.P.Kawatkar et al. Golgi alpha-Mannosidase ii complex with aminocyclopentitetrol. To be published,

Date:

01-Dec-05 Release date: 12-Dec-06

Protein chain

Q24451  (MAN2_DROME) -  Alpha-mannosidase 2

Seq: 1108 a.a.

Struc: 1014 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.2.1.114 - Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase.
• Pathway: Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• Reaction: Hydrolysis of the terminal 1,3- and 1,6-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man(5)(GlcNAc)(3).

 Gene Ontology (GO) functional annotation 

• Cellular component: membrane (6 terms)
• Biological process: metabolic process (3 terms)
• Biochemical function: catalytic activity (11 terms)