PDBsum entry 2f4e

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protein Protein-protein interface(s) links
Signaling protein PDB id
Protein chains
131 a.a. *
Waters ×46
* Residue conservation analysis
PDB id:
Name: Signaling protein
Title: N-terminal domain of fkbp42 from arabidopsis thaliana
Structure: Atfkbp42. Chain: a, b. Fragment: n-terminal fkbp-type domain, residues 1-180. Synonym: twd1 (twisted dwarf 1). Engineered: yes
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Strain: ecotype columbia. Gene: twd1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Biol. unit: Dimer (from PQS)
2.32Å     R-factor:   0.246     R-free:   0.283
Authors: O.H.Weiergraber,A.Eckhoff,J.Granzin
Key ref:
O.H.Weiergräber et al. (2006). Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters. FEBS Lett, 580, 251-255. PubMed id: 16364310 DOI: 10.1016/j.febslet.2005.12.007
23-Nov-05     Release date:   10-Jan-06    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
Q9LDC0  (FKB42_ARATH) -  Peptidyl-prolyl cis-trans isomerase FKBP42
365 a.a.
131 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.  - Peptidylprolyl isomerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Peptidylproline (omega=180) = peptidylproline (omega=0)
Peptidylproline (omega=180)
= peptidylproline (omega=0)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     protein folding   1 term 


    Added reference    
DOI no: 10.1016/j.febslet.2005.12.007 FEBS Lett 580:251-255 (2006)
PubMed id: 16364310  
Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters.
O.H.Weiergräber, A.Eckhoff, J.Granzin.
We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously.
  Selected figure(s)  
Figure 1.
Fig. 1. Superposition of the crystal structures of AtFKBP42^1-180 (blue), HsFKBP12 (grey) and AtFKBP13 (orange). Secondary structure elements of the canonical FKBP fold are numbered consecutively; β0 is an additional β-strand present in AtFKBP42^1-180. The FK506 molecule bound to HsFKBP12 is shown as stick model.
Figure 3.
Fig. 3. Comparison of the molecular surfaces of HsFKBP12 (A) and AtFKBP42^1-180 (B). The FK506 ligand (dark grey) is positioned as in the complex with HsFKBP12. Orientation of the molecules is the same as in Fig. 2.
  The above figures are reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (2006, 580, 251-255) copyright 2006.  
  Figures were selected by the author.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
19366428 D.Meiri, and A.Breiman (2009).
Arabidopsis ROF1 (FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting the accumulation of HsfA2-regulated sHSPs.
  Plant J, 59, 387-399.  
18499676 A.Bailly, V.Sovero, V.Vincenzetti, D.Santelia, D.Bartnik, B.W.Koenig, S.Mancuso, E.Martinoia, and M.Geisler (2008).
Modulation of P-glycoproteins by auxin transport inhibitors is mediated by interaction with immunophilins.
  J Biol Chem, 283, 21817-21826.  
17033777 H.A.Scheidt, A.Vogel, A.Eckhoff, B.W.Koenig, and D.Huster (2007).
Solid-state NMR characterization of the putative membrane anchor of TWD1 from Arabidopsis thaliana.
  Eur Biophys J, 36, 393-404.  
17080288 K.Aviezer-Hagai, J.Skovorodnikova, M.Galigniana, O.Farchi-Pisanty, E.Maayan, S.Bocovza, Y.Efrat, P.von Koskull-Döring, N.Ohad, and A.Breiman (2007).
Arabidopsis immunophilins ROF1 (AtFKBP62) and ROF2 (AtFKBP65) exhibit tissue specificity, are heat-stress induced, and bind HSP90.
  Plant Mol Biol, 63, 237-255.  
17826298 M.Geisler, and A.Bailly (2007).
Tête-à-tête: the function of FKBPs in plant development.
  Trends Plant Sci, 12, 465-473.  
17061141 T.Ishida, S.Thitamadee, and T.Hashimoto (2007).
Twisted growth and organization of cortical microtubules.
  J Plant Res, 120, 61-70.  
  19704625 A.Bailly, V.Sovero, and M.Geisler (2006).
The Twisted Dwarf's ABC: How Immunophilins Regulate Auxin Transport.
  Plant Signal Behav, 1, 277-280.  
16887800 R.Bouchard, A.Bailly, J.J.Blakeslee, S.C.Oehring, V.Vincenzetti, O.R.Lee, I.Paponov, K.Palme, S.Mancuso, A.S.Murphy, B.Schulz, and M.Geisler (2006).
Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins.
  J Biol Chem, 281, 30603-30612.  
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