PDBsum entry: 2f3c

Hydrolase/hydrolase inhibitor

PDB id: 2f3c

Contents

Protein chains

221 a.a. *

46 a.a. *

Ligands

SO4 ×4

Metals

_CA

Waters ×91

* Residue conservation analysis

PDB id:

2f3c

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of infestin 1, a kazal-type serineprotease inhibitor, in complex with trypsin

Structure:

Cationic trypsin. Chain: e. Fragment: residues 21-243. Thrombin inhibitor infestin. Chain: i. Fragment: residues 1-55. Engineered: yes

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Triatoma infestans. Organism_taxid: 30076. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932.

Biol. unit:

Dimer (from PQS)

Resolution:

2.50Å R-factor: 0.170 R-free: 0.252

Authors:

I.T.N.Campos,A.S.Tanaka,J.A.R.G.Barbosa

Key ref:

I.T.N.Campos et al. Crystallographic studies of infestin 1, A thrombin inhibitor from triatoma infestans, In complex to bovine trypsin.. To be published,

Date:

20-Nov-05 Release date: 05-Dec-06

Protein chain

P00760  (TRY1_BOVIN) -  Cationic trypsin

Seq: 246 a.a.

Struc: 221 a.a.

Protein chain

Q95P16  (Q95P16_TRIIF) -  Thrombin inhibitor infestin (Fragment)

Seq: 222 a.a.

Struc: 46 a.a.

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.4 - Trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

 Gene Ontology (GO) functional annotation 

• Cellular component: extracellular region (2 terms)
• Biological process: digestion (2 terms)
• Biochemical function: catalytic activity (7 terms)