PDBsum entry 2f3c

Hydrolase/hydrolase inhibitor

PDB id: 2f3c

Contents

Protein chains

221 a.a. *

46 a.a. *

Ligands

SO4 ×4

Metals

_CA

Waters ×91

* Residue conservation analysis

PDB id:

2f3c

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of infestin 1, a kazal-type serineprotease inhibitor, in complex with trypsin

Structure:

Cationic trypsin. Chain: e. Fragment: residues 21-243. Thrombin inhibitor infestin. Chain: i. Fragment: residues 1-55. Engineered: yes

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Triatoma infestans. Organism_taxid: 30076. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932.

Biol. unit:

Dimer (from PQS)

Resolution:

2.50Å R-factor: 0.170 R-free: 0.252

Authors:

I.T.N.Campos,A.S.Tanaka,J.A.R.G.Barbosa

Key ref:

I.T.Campos et al. (2012). The Kazal-type inhibitors infestins 1 and 4 differ in specificity but are similar in three-dimensional structure. Acta Crystallogr D Biol Crystallogr, 68, 695-702. PubMed id: 22683792

Date:

20-Nov-05 Release date: 05-Dec-06

Protein chain

P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus

Seq: 246 a.a.

Struc: 221 a.a.

Protein chain

Q95P16  (Q95P16_TRIIF) -  Thrombin inhibitor infestin (Fragment) from Triatoma infestans

Seq: 222 a.a.

Struc: 46 a.a.

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Acta Crystallogr D Biol Crystallogr 68:695-702 (2012)

PubMed id: 22683792

The Kazal-type inhibitors infestins 1 and 4 differ in specificity but are similar in three-dimensional structure.

I.T.Campos, T.A.Souza, R.J.Torquato, R.De Marco, A.M.Tanaka-Azevedo, A.S.Tanaka, J.A.Barbosa.

ABSTRACT

No abstract given.