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PDBsum entry 2f28

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protein metals links
Hydrolase PDB id
2f28
Jmol
Contents
Protein chain
358 a.a. *
Metals
_CL ×2
Waters ×276
* Residue conservation analysis
PDB id:
2f28
Name: Hydrolase
Title: Crystal structure of the human sialidase neu2 q116e mutant
Structure: Sialidase 2. Chain: a. Synonym: neu2, cytosolic sialidase, n-acetyl-alpha- neuraminidase 2. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Resolution:
1.67Å     R-factor:   0.216     R-free:   0.240
Authors: L.M.G.Chavas,R.Kato,P.Fusi,C.Tringali,B.Venerando, G.Tettamanti,E.Monti,S.Wakatsuki
Key ref: L.M.G.Chavas et al. Crystal structure of the human sialidase neu2 q116e mutant. To be published, .
Date:
15-Nov-05     Release date:   21-Nov-06    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9Y3R4  (NEUR2_HUMAN) -  Sialidase-2
Seq:
Struc:
380 a.a.
358 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.18  - Exo-alpha-sialidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)-glycosidic linkages of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     metabolic process   9 terms 
  Biochemical function     exo-alpha-(2->3)-sialidase activity     6 terms