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PDBsum entry 2esw

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protein metals Protein-protein interface(s) links
Signaling protein PDB id
2esw
Jmol
Contents
Protein chains
60 a.a. *
57 a.a. *
Metals
_CL ×3
_HG ×6
Waters ×111
* Residue conservation analysis
PDB id:
2esw
Name: Signaling protein
Title: Atomic structure of the n-terminal sh3 domain of mouse beta pix,p21-activated kinase (pak)-interacting exchange factor
Structure: Rho guanine nucleotide exchange factor 7. Chain: a, b. Fragment: sh3 domain. Synonym: pak-interacting exchange factor beta, beta-pix, p85spr. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.01Å     R-factor:   0.232     R-free:   0.254
Authors: Z.Rao
Key ref: X.Li et al. (2006). Crystal structure of the N-terminal SH3 domain of mouse betaPIX, p21-activated kinase-interacting exchange factor. Biochem Biophys Res Commun, 339, 407-414. PubMed id: 16307729 DOI: 10.1016/j.bbrc.2005.10.212
Date:
27-Oct-05     Release date:   11-Apr-06    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9ES28  (ARHG7_MOUSE) -  Rho guanine nucleotide exchange factor 7
Seq:
Struc:
 
Seq:
Struc:
862 a.a.
60 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9ES28  (ARHG7_MOUSE) -  Rho guanine nucleotide exchange factor 7
Seq:
Struc:
 
Seq:
Struc:
862 a.a.
57 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 

 
DOI no: 10.1016/j.bbrc.2005.10.212 Biochem Biophys Res Commun 339:407-414 (2006)
PubMed id: 16307729  
 
 
Crystal structure of the N-terminal SH3 domain of mouse betaPIX, p21-activated kinase-interacting exchange factor.
X.Li, X.Liu, F.Sun, J.Gao, H.Zhou, G.F.Gao, M.Bartlam, Z.Rao.
 
  ABSTRACT  
 
The mouse betaPIX-SH3 domain, residues 8-63 of P21-activated kinase interacting exchange factor, has been characterized by X-ray diffraction. Crystals belonging to space group P3(2)21 diffracted to 2.0 A and the structure was phased by the single-wavelength anomalous diffraction method. The domain is a compact beta-barrel with an overall conformation similar to the general SH3 structure. The X-ray structure shows mouse betaPIX-SH3 domain binding the way in which the betaPIX characteristic amino acids do so for an unconventional ligand binding surface. This arrangement provides a rationale for the unusual ligand recognition motif exhibited by mouse betaPIX-SH3 domain. Comparison with another SH3/peptide complex shows that the recognition mode of the mouse betaPIX-SH3 domain should be very similar to the RXXK ligand binding mode. The unique large and planar hydrophobic pocket may contribute to the promiscuity of betaPIX-SH3 domain resulting in its multiple biological functions.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
16983070 C.Gu, S.G.Tangye, X.Sun, Y.Luo, Z.Lin, and J.Wu (2006).
The X-linked lymphoproliferative disease gene product SAP associates with PAK-interacting exchange factor and participates in T cell activation.
  Proc Natl Acad Sci U S A, 103, 14447-14452.  
16857672 X.Li, Y.Chen, Y.Liu, J.Gao, F.Gao, M.Bartlam, J.Y.Wu, and Z.Rao (2006).
Structural basis of Robo proline-rich motif recognition by the srGAP1 Src homology 3 domain in the Slit-Robo signaling pathway.
  J Biol Chem, 281, 28430-28437.
PDB code: 2gnc
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.