PDBsum entry 2eb7

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protein links
Transcription PDB id
Protein chain
141 a.a. *
Waters ×115
* Residue conservation analysis
PDB id:
Name: Transcription
Title: Crystal structure of the hypothetical regulator from sulfolo tokodaii 7
Structure: 146aa long hypothetical transcriptional regulator chain: a. Engineered: yes
Source: Sulfolobus tokodaii. Organism_taxid: 273063. Strain: 7. Gene: st1710. Expressed in: escherichia coli. Expression_system_taxid: 562.
1.80Å     R-factor:   0.228     R-free:   0.268
Authors: T.S.Kumarevel,M.Nishio,A.Shinkai,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref: T.Kumarevel et al. (2008). Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. J Struct Biol, 161, 9. PubMed id: 17933554 DOI: 10.1016/j.jsb.2007.08.017
07-Feb-07     Release date:   06-Nov-07    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
Q96ZY1  (Q96ZY1_SULTO) -  Putative MarR family transcriptional regulator
146 a.a.
141 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   1 term 
  Biological process     transcription, DNA-dependent   2 terms 
  Biochemical function     DNA binding     3 terms  


DOI no: 10.1016/j.jsb.2007.08.017 J Struct Biol 161:9 (2008)
PubMed id: 17933554  
Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7.
T.Kumarevel, T.Tanaka, M.Nishio, S.C.Gopinath, K.Takio, A.Shinkai, P.K.Kumar, S.Yokoyama.
The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.

Literature references that cite this PDB file's key reference

  PubMed id Reference
  19255465 C.E.Nichols, S.Sainsbury, J.Ren, T.S.Walter, A.Verma, D.K.Stammers, N.J.Saunders, and R.J.Owens (2009).
The structure of NMB1585, a MarR-family regulator from Neisseria meningitidis.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 65, 204-209.
PDB code: 3g3z
19509310 T.Kumarevel, T.Tanaka, T.Umehara, and S.Yokoyama (2009).
ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.
  Nucleic Acids Res, 37, 4723-4735.
PDB codes: 3gez 3gf2 3gfi 3gfj 3gfl 3gfm
18812515 M.S.Wilke, M.Heller, A.L.Creagh, C.A.Haynes, L.P.McIntosh, K.Poole, and N.C.Strynadka (2008).
The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR.
  Proc Natl Acad Sci U S A, 105, 14832-14837.
PDB code: 3ech
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.