PDBsum entry 2dw1

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protein ligands metals Protein-protein interface(s) links
Apoptosis, toxin PDB id
Protein chains
415 a.a. *
GM6 ×2
_CA ×6
_ZN ×2
Waters ×211
* Residue conservation analysis
PDB id:
Name: Apoptosis, toxin
Title: Crystal structure of vap2 from crotalus atrox venom (form 2-
Structure: Catrocollastatin. Chain: a, b. Fragment: residues 191-609. Synonym: vap2, vascular apoptosis-inducing protein-2, catrocollastatin/vap2b
Source: Crotalus atrox. Western diamondback rattlesnake. Organism_taxid: 8730
2.50Å     R-factor:   0.227     R-free:   0.286
Authors: S.Takeda,T.Igarashi,S.Araki
Key ref: T.Igarashi et al. (2007). Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family proteins. FEBS Lett, 581, 2416-2422. PubMed id: 17485084 DOI: 10.1016/j.febslet.2007.04.057
02-Aug-06     Release date:   10-Jul-07    
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Protein chains
Pfam   ArchSchema ?
Q90282  (VM3VB_CROAT) -  Zinc metalloproteinase-disintegrin-like VAP2B
609 a.a.
415 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     metallopeptidase activity     2 terms  


DOI no: 10.1016/j.febslet.2007.04.057 FEBS Lett 581:2416-2422 (2007)
PubMed id: 17485084  
Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family proteins.
T.Igarashi, S.Araki, H.Mori, S.Takeda.
Catrocollastatin/vascular apoptosis-inducing protein (VAP)2B is a metalloproteinase from Crotalus atrox venom, possessing metalloproteinase/disintegrin/cysteine-rich (MDC) domains that bear the typical domain architecture of a disintegrin and metalloproteinase (ADAM)/adamalysin/reprolysin family proteins. Here we describe crystal structures of catrocollastatin/VAP2B in three different crystal forms, representing the first reported crystal structures of a member of the monomeric class of this family of proteins. The overall structures show good agreement with both monomers of atypical homodimeric VAP1. Comparison of the six catrocollastatin/VAP2B monomer structures and the structures of VAP1 reveals a dynamic, modular architecture that may be important for the functions of ADAM/adamalysin/reprolysin family proteins.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21333669 A.Pinyachat, P.Rojnuckarin, C.Muanpasitporn, P.Singhamatr, and S.Nuchprayoon (2011).
Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
  Toxicon, 57, 772-780.  
21415364 C.J.Tape, S.H.Willems, S.L.Dombernowsky, P.L.Stanley, M.Fogarasi, W.Ouwehand, J.McCafferty, and G.Murphy (2011).
Cross-domain inhibition of TACE ectodomain.
  Proc Natl Acad Sci U S A, 108, 5578-5583.  
21255601 T.Teklemariam, A.I.Seoane, C.J.Ramos, E.E.Sanchez, S.E.Lucena, J.C.Perez, S.A.Mandal, and J.G.Soto (2011).
Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells.
  Toxicon, 57, 646-656.  
19692335 H.Liu, A.H.Shim, and X.He (2009).
Structural characterization of the ectodomain of a disintegrin and metalloproteinase-22 (ADAM22), a neural adhesion receptor instead of metalloproteinase: insights on ADAM function.
  J Biol Chem, 284, 29077-29086.  
19643179 L.Troeberg, K.Fushimi, S.D.Scilabra, H.Nakamura, V.Dive, I.B.Thøgersen, J.J.Enghild, and H.Nagase (2009).
The C-terminal domains of ADAMTS-4 and ADAMTS-5 promote association with N-TIMP-3.
  Matrix Biol, 28, 463-469.  
18706512 S.Takeda (2009).
Three-dimensional domain architecture of the ADAM family proteinases.
  Semin Cell Dev Biol, 20, 146-152.  
18344021 A.Zolkiewska (2008).
ADAM proteases: ligand processing and modulation of the Notch pathway.
  Cell Mol Life Sci, 65, 2056-2068.  
18479462 J.W.Fox, and S.M.Serrano (2008).
Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity.
  FEBS J, 275, 3016-3030.  
18391413 N.Moiseeva, R.Bau, S.D.Swenson, F.S.Markland, J.Y.Choe, Z.J.Liu, and M.Allaire (2008).
Structure of acostatin, a dimeric disintegrin from Southern copperhead (Agkistrodon contortrix contortrix), at 1.7 A resolution.
  Acta Crystallogr D Biol Crystallogr, 64, 466-470.
PDB code: 3c05
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