PDBsum entry: 2d0q

Lyase

PDB id: 2d0q

Contents

Protein chains

192 a.a. *

212 a.a. *

Ligands

CYI

Metals

_MG ×6

_FE

Waters ×585

* Residue conservation analysis

PDB id:

2d0q

Name:

Lyase

Title:

Complex of fe-type nhase with cyclohexyl isocyanide, photo- activated for 1hr at 277k

Structure:

Nitrile hydratase subunit alpha. Chain: a. Synonym: nitrilase, nhase. Nitrile hydratase subunit beta. Chain: b. Synonym: nitrilase, nhase. Ec: 4.2.1.84

Source:

Rhodococcus erythropolis. Organism_taxid: 1833. Strain: n771. Strain: n771

Biol. unit:

Dimer (from PQS)

Resolution:

1.65Å R-factor: 0.154 R-free: 0.165

Authors:

M.Nojiri,Y.Kawano,K.Hashimoto,N.Kamiya,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

M.Nojiri et al. X-Ray snap shots of inhibitor binding process in photo-Reactive nitrile hydratase. To be published,

Date:

05-Aug-05 Release date: 05-Feb-06

Protein chain

P13448  (NHAA_RHOER) -  Nitrile hydratase subunit alpha

Seq: 207 a.a.

Struc: 192 a.a.*

Protein chain

P13449  (NHAB_RHOER) -  Nitrile hydratase subunit beta

Seq: 212 a.a.

Struc: 212 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.4.2.1.84 - Nitrile hydratase.
• Reaction: An aliphatic amide = a nitrile + H₂O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: plastid (1 term)
• Biological process: nitrogen compound metabolic process (2 terms)
• Biochemical function: catalytic activity (6 terms)