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Hydrolase PDB id
2bzl
Jmol
Contents
Protein chain
284 a.a. *
Ligands
SO4 ×2
EDO ×2
Waters ×244
* Residue conservation analysis
PDB id:
2bzl
Name: Hydrolase
Title: Crystal structure of the human protein tyrosine phosphatase n14 at 1.65 a resolution
Structure: Tyrosine-protein phosphatase, non-receptor type 14. Chain: a. Fragment: catalytic domain, residues 886-1187. Synonym: protein-tyrosine phosphatase pez. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_cell_line: rosetta.
Resolution:
1.65Å     R-factor:   0.186     R-free:   0.217
Authors: J.E.Debreczeni,A.Barr,J.Eswaran,S.Das,N.Burgess,E.Longman, O.Fedorov,O.Gileadi,F.Von Delft,M.Sundstrom,C.Arrowsmith, J.Weigelt,A.Edwards,S.Knapp
Key ref:
A.J.Barr et al. (2006). Crystal structure of human protein tyrosine phosphatase 14 (PTPN14) at 1.65-A resolution. Proteins, 63, 1132-1136. PubMed id: 16534812 DOI: 10.1002/prot.20958
Date:
18-Aug-05     Release date:   13-Sep-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q15678  (PTN14_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 14
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1187 a.a.
284 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.48  - Protein-tyrosine-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate
Protein tyrosine phosphate
 + H(2)O
 = protein tyrosine
 + phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     dephosphorylation   2 terms 
  Biochemical function     phosphatase activity     2 terms  

 

 
    reference    
 
 
DOI no: 10.1002/prot.20958 Proteins 63:1132-1136 (2006)
PubMed id: 16534812  
 
 
Crystal structure of human protein tyrosine phosphatase 14 (PTPN14) at 1.65-A resolution.
A.J.Barr, J.E.Debreczeni, J.Eswaran, S.Knapp.
 
  ABSTRACT  
 
No abstract given.

 
  Selected figure(s)  
 
Figure 1.
Figure 1. (A) Three-dimensional structure of PTPN14. Secondary structure elements were determined using the program ICM (Molsoft) and labeled using the nomenclature in ref.[19]. The WPD loop is shown in yellow, -helices are in red, -strands in green, and the 3[10]-helices in magenta. The active site Cys1121 and cocrystallizing sulfate ions are shown as a ball-and-stick representation. (B) Structure-based sequence alignment of PTPN14 and PTP1B (PDB code: 2HNP). Secondary structure elements are colored as above. 		Figure 2.
Figure 2. Superimposition of PTP1B (PDB code: 2HNP) colored red and PTPN14 (PDB code: 2BZL) in yellow showing the extended loop. The phosphate binding loop of the active site is shown in blue for orientation and the position of the Thr1068/Met mutation is shown.
 
  The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2006, 63, 1132-1136) copyright 2006.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
19167335 A.J.Barr, E.Ugochukwu, W.H.Lee, O.N.King, P.Filippakopoulos, I.Alfano, P.Savitsky, N.A.Burgess-Brown, S.Müller, and S.Knapp (2009).
Large-scale structural analysis of the classical human protein tyrosine phosphatome.
  Cell, 136, 352-363.
PDB codes: 2ahs 2b49 2gjt 2i75 2nlk 2nz6 2oc3 2ooq 2p6x 2pa5 2qep 3b7o
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