PDBsum entry: 2bsp

Lyase

PDB id: 2bsp

Contents

Protein chain

399 a.a. *

Metals

_CA

Waters ×246

* Residue conservation analysis

PDB id:

2bsp

Name:

Lyase

Title:

Bacillus subtilis pectate lyase r279k mutant

Structure:

Protein (pectate lyase). Chain: a. Engineered: yes. Mutation: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Strain: s1103. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.196 R-free: 0.225

Authors:

R.Pickersgill

Key ref:

R.Pickersgill et al. The conserved arginine proximal to the essential calcium of bacillus subtilis pectate lyase stabilizes the transition state. To be published,

Date:

31-Jul-98 Release date: 12-Aug-98

Protein chain

P39116  (PLY_BACSU) -  Pectate lyase

Seq: 420 a.a.

Struc: 399 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.2.2 - Pectate lyase.
• Pathway: Pectin and Pectate Lyases
• Reaction: Eliminative cleavage of pectate to give oligosaccharides with 4-deoxy- alpha-D-gluc-4-enuronosyl groups at their non-reducing ends.