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PDBsum entry 2bpi

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protein metals Protein-protein interface(s) links
Oxidoreductase PDB id
2bpi
Jmol
Contents
Protein chains
197 a.a. *
Metals
_FE ×2
Waters ×140
* Residue conservation analysis
PDB id:
2bpi
Name: Oxidoreductase
Title: Structure of iron dependent superoxide dismutase from p. Fal
Structure: Fe-superoxide dismutase. Chain: a, b. Engineered: yes
Source: Plasmodium falciparum. Organism_taxid: 36329. Strain: 3d7. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Resolution:
2.52Å     R-factor:   0.187     R-free:   0.263
Authors: I.W.Boucher,J.A.Brannigan,A.J.Wilkinson,A.M.Brzozowski
Key ref: I.W.Boucher et al. (2006). The crystal structure of superoxide dismutase from Plasmodium falciparum. BMC Struct Biol, 6, 20. PubMed id: 17020617
Date:
20-Apr-05     Release date:   11-Oct-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q8IAY6  (SODF_PLAF7) -  Superoxide dismutase [Fe]
Seq:
Struc:
198 a.a.
197 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.1.15.1.1  - Superoxide dismutase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 2 superoxide + 2 H+ = O2 + H2O2
2 × superoxide
+ 2 × H(+)
= O(2)
+ H(2)O(2)
      Cofactor: Fe cation or Mn(2+) or (Zn(2+) and Cu cation)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     oxidation-reduction process   4 terms 
  Biochemical function     protein binding     4 terms  

 

 
    Added reference    
 
 
BMC Struct Biol 6:20 (2006)
PubMed id: 17020617  
 
 
The crystal structure of superoxide dismutase from Plasmodium falciparum.
I.W.Boucher, A.M.Brzozowski, J.A.Brannigan, C.Schnick, D.J.Smith, S.A.Kyes, A.J.Wilkinson.
 
  ABSTRACT  
 
BACKGROUND: Superoxide dismutases (SODs) are important enzymes in defence against oxidative stress. In Plasmodium falciparum, they may be expected to have special significance since part of the parasite life cycle is spent in red blood cells where the formation of reactive oxygen species is likely to be promoted by the products of haemoglobin breakdown. Thus, inhibitors of P. falciparum SODs have potential as anti-malarial compounds. As a step towards their development we have determined the crystal structure of the parasite's cytosolic iron superoxide dismutase. RESULTS: The cytosolic iron superoxide dismutase from P. falciparum (PfFeSOD) has been overexpressed in E. coli in a catalytically active form. Its crystal structure has been solved by molecular replacement and refined against data extending to 2.5 A resolution. The structure reveals a two-domain organisation and an iron centre in which the metal is coordinated by three histidines, an aspartate and a solvent molecule. Consistent with ultracentrifugation analysis the enzyme is a dimer in which a hydrogen bonding lattice links the two active centres. CONCLUSION: The tertiary structure of PfFeSOD is very similar to those of a number of other iron-and manganese-dependent superoxide dismutases, moreover the active site residues are conserved suggesting a common mechanism of action. Comparison of the dimer interfaces of PfFeSOD with the human manganese-dependent superoxide dismutase reveals a number of differences, which may underpin the design of parasite-selective superoxide dismutase inhibitors.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19384994 J.F.Bachega, M.V.Navarro, L.Bleicher, R.K.Bortoleto-Bugs, D.Dive, P.Hoffmann, E.Viscogliosi, and R.C.Garratt (2009).
Systematic structural studies of iron superoxide dismutases from human parasites and a statistical coupling analysis of metal binding specificity.
  Proteins, 77, 26-37.
PDB codes: 2goj 2gpc 3esf
19827093 S.E.Dobson, K.D.Augustijn, J.A.Brannigan, C.Schnick, C.J.Janse, E.J.Dodson, A.P.Waters, and A.J.Wilkinson (2009).
The crystal structures of macrophage migration inhibitory factor from Plasmodium falciparum and Plasmodium berghei.
  Protein Sci, 18, 2578-2591.
PDB codes: 2wkb 2wkf
18551319 J.M.Kang, H.I.Cheun, J.Kim, S.U.Moon, S.J.Park, T.S.Kim, W.M.Sohn, and B.K.Na (2008).
Identification and characterization of a mitochondrial iron-superoxide dismutase of Cryptosporidium parvum.
  Parasitol Res, 103, 787-795.  
17875391 P.Gayathri, H.Balaram, and M.R.Murthy (2007).
Structural biology of plasmodial proteins.
  Curr Opin Struct Biol, 17, 744-754.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.