PDBsum entry 2b97

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protein metals Protein-protein interface(s) links
Surface active protein PDB id
Protein chains
70 a.a. *
Waters ×256
* Residue conservation analysis
PDB id:
Name: Surface active protein
Title: Ultra-high resolution structure of hydrophobin hfbii
Structure: Hydrophobin ii. Chain: a, b. Synonym: hfbii
Source: Hypocrea jecorina. Organism_taxid: 51453
Biol. unit: Tetramer (from PQS)
0.75Å     R-factor:   0.130     R-free:   0.148
Authors: J.Hakanpaa,M.Linder,A.Popov,A.Schmidt,J.Rouvinen
Key ref:
J.Hakanpää et al. (2006). Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 A. Acta Crystallogr D Biol Crystallogr, 62, 356-367. PubMed id: 16552136 DOI: 10.1107/S0907444906000862
11-Oct-05     Release date:   28-Mar-06    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P79073  (HYP2_HYPJE) -  Hydrophobin-2
86 a.a.
70 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   3 terms 


DOI no: 10.1107/S0907444906000862 Acta Crystallogr D Biol Crystallogr 62:356-367 (2006)
PubMed id: 16552136  
Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 A.
J.Hakanpää, M.Linder, A.Popov, A.Schmidt, J.Rouvinen.
Hydrophobins are small proteins secreted by filamentous fungi that have a unique ability to spontaneously form amphiphilic layers. Hydrophobins have only recently been structurally characterized through the first crystal structure determination of a protein of this class, Trichoderma reesei hydrophobin HFBII [Hakanpää, Paananen et al. (2004), J. Biol. Chem. 279, 534-539]. The resolution of the HFBII structure has now been extended to an ultrahigh resolution of 0.75 A. The structure was refined conventionally and multipole refinement has been initiated. The ultrahigh-resolution structure is analyzed here in detail and comparison is made to the previous atomic resolution structure of the same protein as well as to other ultrahigh-resolution structures found in the Protein Data Bank.
  Selected figure(s)  
Figure 1.
Figure 1 (a) Sequence of T. reesei hydrophobin HFBII, with yellow colour indicating the conserved cysteine residues and red colour highlighting the residues of the hydrophobic patch. The signal sequence is shown in blue. (b) The structure of T. reeesei hydrophobin HFBII; disulfide bridges are highlighted in yellow and the hydrophobic patch in red. The figure was produced using PyMOL (DeLano, 2002[66] [DeLano, W. L. (2002). The PyMOL Molecular Graphics System. DeLano Scientific, CA, USA. .]-[67][bluearr.gif] ).
Figure 3.
Figure 3 Omit map of HFBII ultrahigh-resolution structure showing density at the hydrogen positions of Ile22, chain B. The figure was produced using PyMOL (DeLano, 2002[137] [DeLano, W. L. (2002). The PyMOL Molecular Graphics System. DeLano Scientific, CA, USA. .]-[138][bluearr.gif] ). 2F[o] - F[c] maps at 1.0 [139][sigma] are shown in blue and F[o] - F[c] maps at 2.0 [140][sigma] in pink.
  The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2006, 62, 356-367) copyright 2006.  
  Figures were selected by the author.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
20100692 A.Armenante, S.Longobardi, I.Rea, L.De Stefano, M.Giocondo, A.Silipo, A.Molinaro, and P.Giardina (2010).
The Pleurotus ostreatus hydrophobin Vmh2 and its interaction with glucans.
  Glycobiology, 20, 594-602.  
20615601 A.Cooper, and M.W.Kennedy (2010).
Biofoams and natural protein surfactants.
  Biophys Chem, 151, 96.  
20445231 A.S.Gardberg, A.R.Del Castillo, K.L.Weiss, F.Meilleur, M.P.Blakeley, and D.A.Myles (2010).
Unambiguous determination of H-atom positions: comparing results from neutron and high-resolution X-ray crystallography.
  Acta Crystallogr D Biol Crystallogr, 66, 558-567.
PDB codes: 3kyu 3kyv 3kyw 3kyx 3kyy
18814299 P.Labute (2009).
Protonate3D: assignment of ionization states and hydrogen coordinates to macromolecular structures.
  Proteins, 75, 187-205.  
18053245 D.M.Lemaster, J.S.Anderson, L.Wang, Y.Guo, H.Li, and G.Hernandez (2007).
NMR and X-ray analysis of structural additivity in metal binding site-swapped hybrids of rubredoxin.
  BMC Struct Biol, 7, 81.
PDB codes: 2pve 2pvx
17636262 J.M.Kallio, M.B.Linder, and J.Rouvinen (2007).
Crystal structures of hydrophobin HFBII in the presence of detergent implicate the formation of fibrils and monolayer films.
  J Biol Chem, 282, 28733-28739.
PDB codes: 2pl6 2pl7
16882996 J.Hakanpää, G.R.Szilvay, H.Kaljunen, M.Maksimainen, M.Linder, and J.Rouvinen (2006).
Two crystal structures of Trichoderma reesei hydrophobin HFBI--the structure of a protein amphiphile with and without detergent interaction.
  Protein Sci, 15, 2129-2140.
PDB codes: 2fz6 2gvm
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