PDBsum entry: 2b8o

Hydrolase/hydrolase inhibitor

PDB id: 2b8o

Contents

Protein chains

142 a.a. *

254 a.a. *

191 a.a. *

Ligands

GLC

FUC

0GJ

Metals

_CA ×6

_CL ×3

_MG ×2

_NA

_ZN ×2

Waters ×239

* Residue conservation analysis

PDB id:

2b8o

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of glu-gly-arg-chloromethyl ketone-factor viia/soluble tissue factor complex

Structure:

Coagulation factor vii light chain. Chain: l. Engineered: yes. Coagulation factor vii heavy chain. Chain: h. Engineered: yes. Tissue factor. Chain: t. Synonym: tf, coagulation factor iii, thromboplastin, cd142

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: f7. Gene: f3

Resolution:

2.80Å R-factor: 0.225 R-free: 0.285

Authors:

S.P.Bajaj,A.E.Schmidt,K.Padmanabhan

Key ref:

S.P.Bajaj et al. Crystal structure of factor viia/soluble tissue factor complexed with glu-Gly-Arg-Chloromethyl ketone. To be published,

Date:

08-Oct-05 Release date: 13-Feb-07

Protein chain

P08709  (FA7_HUMAN) -  Coagulation factor VII

Seq: 466 a.a.

Struc: 142 a.a.*

Protein chain

P08709  (FA7_HUMAN) -  Coagulation factor VII

Seq: 466 a.a.

Struc: 254 a.a.

Protein chain

P13726  (TF_HUMAN) -  Tissue factor

Seq: 295 a.a.

Struc: 191 a.a.

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains L, H: E.C.3.4.21.21 - Coagulation factor VIIa.
• Reaction: Hydrolyzes one Arg-|-Ile bond in factor X to form factor Xa.

 Gene Ontology (GO) functional annotation 

• Cellular component: extracellular region (2 terms)
• Biological process: blood coagulation (2 terms)
• Biochemical function: catalytic activity (4 terms)