PDBsum entry: 2ash

Transferase

PDB id: 2ash

Contents

Protein chains

361 a.a. *

Ligands

EDO ×10

Metals

_ZN ×4

_CL ×5

Waters ×655

* Residue conservation analysis

PDB id:

2ash

Name:

Transferase

Title:

Crystal structure of queuine tRNA-ribosyltransferase (ec 2.4 (tRNA-guanine (tm1561) from thermotoga maritima at 1.90 a r

Structure:

Queuine tRNA-ribosyltransferase. Chain: a, b, c, d. Synonym: tRNA-guanine transglycosylase, guanine insertion e engineered: yes

Source:

Thermotoga maritima. Organism_taxid: 2336. Gene: tgt. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.90Å R-factor: 0.193 R-free: 0.229

Authors:

Joint Center For Structural Genomics (Jcsg)

Key ref:

Joint center for structural genomics (jcsg) Crystal structure of queuine tRNA-Ribosyltransferase (EC 2.4.2.29) (tRNA-Guanine (tm1561) from thermotoga maritima at 1.90 a resolution. To be published,

Date:

23-Aug-05 Release date: 06-Sep-05

Protein chains

Q9X1P7  (TGT_THEMA) -  Queuine tRNA-ribosyltransferase

Seq: 369 a.a.

Struc: 361 a.a.

Enzyme reactions

• Enzyme class: E.C.2.4.2.29 - tRNA-guanine transglycosylase.
• Reaction:
  1. [tRNA]-guanine + queuine = [tRNA]-queuine + guanine
  2. [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl- 7-carbaguanine + guanine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: tRNA processing (3 terms)
• Biochemical function: transferase activity (4 terms)