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110 a.a.
Key reference
DOI no: 10.1110/ps.051736805 Protein Sci 14:2895-2900 (2005) PubMed id: 16199667
Crystal structure of the N-terminal domain of E. coli Lon protease. M.Li, F.Rasulova, E.E.Melnikov, T.V.Rotanova, A.Gustchina, M.R.Maurizi, A.Wlodawer. ABSTRACT
We report here the first crystal structure of the N-terminal domain of an A-type Lon protease. Lon proteases are ubiquitous, multidomain, ATP-dependent enzymes with both highly specific and non-specific protein binding, unfolding, and degrading activities. We expressed and purified a stable, monomeric 119-amino acid N-terminal subdomain of the Escherichia coli A-type Lon protease and code 2ANE). The structure was solved in two crystal forms, yielding 14 independent views. The domain exhibits a unique fold consisting primarily of three twisted beta-sheets and a single long alpha-helix. Analysis of recent PDB depositions identified a similar fold in BPP1347 (PDB code 1ZBO), a 203-amino acid protein of unknown function from Bordetella parapertussis, crystallized as part of a structural genomics effort. BPP1347 shares sequence homology with Lon N-domains and with a family of other independently expressed proteins of unknown functions. We postulate that, as is the case in Lon proteases, this structural domain represents a general protein and polypeptide interaction domain.
Selected figure(s)
Figure 1.
Figure 1. Crystal structure of the N119 domain of E. coli Lon and its comparison with the structure of the hypothetical protein BPP1347. (A) Stereo view of LonN119 showing the elements of the secondary structure colored green forstrands, blue for
helices, and brown for coils. (B) Superposition of LonN119 (green) and B. parapertussis BPP1347 (gray).
The above figure is reprinted by permission from the Protein Society: Protein Sci (2005, 14, 2895-2900) copyright 2005. Figure was selected by the author.
Literature references that cite this PDB file's key reference
PubMed id Reference
18446870 B.Friguet, A.L.Bulteau, and I.Petropoulos (2008).
Mitochondrial protein quality control: implications in ageing.Biotechnol J, 3, 757-764.
18441029 M.Chruszcz, A.Wlodawer, and W.Minor (2008).
Determination of protein structures--a series of fortunate events.Biophys J, 95, 1-9. 16511295 T.Arakaki, I.Le Trong, E.Phizicky, E.Quartley, G.DeTitta, J.Luft, A.Lauricella, L.Anderson, O.Kalyuzhniy, E.Worthey, P.J.Myler, D.Kim, D.Baker, W.G.Hol, and E.A.Merritt (2006).
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 175-179.
PDB code: 2ar1
16877706 T.V.Rotanova, I.Botos, E.E.Melnikov, F.Rasulova, A.Gustchina, M.R.Maurizi, and A.Wlodawer (2006).
Slicing a protease: structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains.Protein Sci, 15, 1815-1828. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.
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