PDBsum entry: 2alw

Hydrolase

PDB-id: 2alw

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Description

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PROCHECK

Protein chain

1014 a.a. *

Ligands

NAG

MNM

MPD

Metal ions

_ZN

Waters ×1073

* Residue conservation analysis

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PDB id:

2alw

Name:

Hydrolase

Title:

Golgi alpha-mannosidase ii complex with noeuromycin

Structure:

Alpha-mannosidase ii. Chain: a. Fragment: catalytic domain. Synonym: mannosyl-oligosaccharide 1,3-1,6- alpha- mannosidase, man ii, golgi alpha-mannosidase ii, aman ii. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: gmii. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: s2 cells.

UniProt:

Q24451 (MAN2_DROME)

Seq:

Struc:

Seq:

Struc:

Seq:

Struc:

Seq:

Struc:

Seq:

1108 a.a.

Struc:

1014 a.a.*

Key:	PfamA domain	PfamB domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme class:

E.C.3.2.1.114   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Hydrolysis of the terminal 1,3- and 1,6-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man(5)(GlcNAc)(3).

Pathway:

Mannosyl-glycoprotein N-acetylglucosaminyltransferases

Resolution:

1.86Å

R-factor:

0.141

R-free:

0.181

Authors:

D.A.Kuntz,M.B.Bols,H.Liu,D.R.Rose

Key ref:

. [PubMed id: ] d.a.kuntz et al. (2006). The role of the active site Zn in the catalytic mechanism of the GH38 Golgi alpha-mannosidase II: implications from noeuromycin inhibition. Biocatal.Biotransfor., 34, 55-61.

Date:

08-Aug-05

Release date:

04-Jul-06

Related entries:

1hty
1hww
1hxk
1ps2
1qwn
1qx1
1r33
1r34
1tqs
1tqt
... plus others (see Header records)

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