PDBsum entry 2a5z

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protein metals Protein-protein interface(s) links
Structural genomics, unknown function PDB id
Protein chains
239 a.a.
_MG ×3
Waters ×555
PDB id:
Name: Structural genomics, unknown function
Title: Crystal structure of protein of unknown function so2946 from shewanella oneidensis mr-1
Structure: Hypothetical protein so2946. Chain: a, b, c. Engineered: yes
Source: Shewanella oneidensis. Organism_taxid: 211586. Strain: mr-1. Gene: so2946. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Trimer (from PQS)
2.02Å     R-factor:   0.168     R-free:   0.203
Authors: B.Nocek,L.Bigelow,J.Abdullah,F.Collart,A.Joachimiak,Midwest For Structural Genomics (Mcsg)
Key ref: B.Nocek et al. (2008). Structure of SO2946 orphan from Shewanella oneidensis shows "jelly-roll" fold with carbohydrate-binding module. J Struct Funct Genomics, 9, 1-6. PubMed id: 18566914
01-Jul-05     Release date:   16-Aug-05    
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Protein chains
Pfam   ArchSchema ?
Q8ED25  (Q8ED25_SHEON) -  Lambda phage protein with carbohydrate-binding module
262 a.a.
239 a.a.
Key:    PfamB domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cellular_component   1 term 
  Biological process     biological_process   1 term 
  Biochemical function     molecular_function     2 terms  


J Struct Funct Genomics 9:1-6 (2008)
PubMed id: 18566914  
Structure of SO2946 orphan from Shewanella oneidensis shows "jelly-roll" fold with carbohydrate-binding module.
B.Nocek, L.Bigelow, J.Abdullah, A.Joachimiak.
The crystal structure of the uncharacterized protein SO2946 from Shewanella oneidensis MR-1 was determined with single-wavelength anomalous diffraction (SAD) and refined to 2.0 A resolution. The SO2946 protein consists of a short helical N-terminal domain and a large C-terminal domain with the "jelly-roll" topology. The protein assembles into a propeller consisting of three C-terminal blades arranged around a central core formed by the N-terminal domains. The function of SO2946 could not be inferred from the sequence since the protein represents an orphan with no sequence homologs, but the protein's structure bears a fold similar to that of proteins containing carbohydrate-binding modules. Features such as fold conservation, the presence of a conserved groove and a metal binding region are indicative that SO2946 may be an enzyme and could be involved in binding carbohydrate molecules.