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Endoglucanase
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PDB id
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2a39
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Contents |
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* Residue conservation analysis
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PDB id:
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Endoglucanase
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Title:
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Humicola insolens endocellulase egi native structure
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Structure:
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Endoglucanase i. Chain: a, b. Synonym: cellulase, family 7 endoglucanase. Engineered: yes. Other_details: pyroglutamate post-translational modificatio residue 1 n-linked n-acetylglucosamine on residue asn 247
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Source:
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Humicola insolens. Organism_taxid: 34413. Gene: potential. Expressed in: aspergillus oryzae. Expression_system_taxid: 5062
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Resolution:
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2.20Å
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R-factor:
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0.180
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R-free:
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0.230
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Authors:
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G.J.Davies,G.Sulzenbacher,L.Mackenzie,S.G.Withers,C.Divne,T. H.F.Woldike,M.Schulein
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Key ref:
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L.F.MacKenzie
et al.
(1998).
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate.
Biochem J,
335,
409-416.
PubMed id:
Ref:
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Date:
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30-Jan-98
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Release date:
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16-Feb-99
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PROCHECK
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Headers
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References
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P56680
(GUN1_HUMIN) -
Endoglucanase 1
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Seq:
Struc:
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402 a.a.
398 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.2.1.4
- Cellulase.
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Reaction:
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Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
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Gene Ontology (GO) functional annotation
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Cellular component
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extracellular region
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1 term
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Biological process
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metabolic process
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4 terms
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Biochemical function
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hydrolase activity
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4 terms
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Biochem J
335:409-416
(1998)
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PubMed id:
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Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate.
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L.F.MacKenzie,
G.Sulzenbacher,
C.Divne,
T.A.Jones,
H.F.Wöldike,
M.Schülein,
S.G.Withers,
G.J.Davies.
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ABSTRACT
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Cellulose is the major polysaccharide component of the plant cell wall and the
most abundant naturally produced macromolecule on Earth. The enzymic degradation
of cellulose, by cellulases, is therefore of great environmental and commercial
significance. Cellulases are found in 12 of the glycoside hydrolase families
classified according to their amino acid sequence similarities. Endoglucanase I
(Cel7B), from the soft-rot fungus Humicola insolens, is a family 7 enzyme. The
structure of the native form of Cel7B from H. insolens at 2.2 A resolution has
been solved by molecular replacement using the known Trichoderma reesei
cellobiohydrolase I [Divne, Ståhlberg, Reinikainen, Ruohonen, Pettersson,
structure as the search
model. Cel7B catalyses hydrolysis of the beta-1,4 glycosidic linkages in
cellulose with net retention of anomeric configuration. The catalytic
nucleophile at the active site of Cel7B has been identified as Glu-197 by
trapping of a 2-deoxy-2-fluorocellotriosyl enzyme intermediate and
identification of the labelled peptide in peptic digests by tandem MS.
Site-directed mutagenesis of both Glu-197 and the prospective catalytic acid,
Glu-202, results in inactive enzyme, confirming the critical role of these
groups for catalysis.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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T.Parkkinen,
A.Koivula,
J.Vehmaanperä,
and
J.Rouvinen
(2008).
Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding.
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Protein Sci, 17,
1383-1394.
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PDB codes:
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C.Mulakala,
and
P.J.Reilly
(2005).
Force calculations in automated docking: enzyme-substrate interactions in Fusarium oxysporum Cel7B.
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Proteins, 61,
590-596.
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W.Ubhayasekera,
I.G.Muñoz,
A.Vasella,
J.Ståhlberg,
and
S.L.Mowbray
(2005).
Structures of Phanerochaete chrysosporium Cel7D in complex with product and inhibitors.
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FEBS J, 272,
1952-1964.
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PDB codes:
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J.Allouch,
W.Helbert,
B.Henrissat,
and
M.Czjzek
(2004).
Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose.
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Structure, 12,
623-632.
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PDB code:
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M.F.Amaya,
A.G.Watts,
I.Damager,
A.Wehenkel,
T.Nguyen,
A.Buschiazzo,
G.Paris,
A.C.Frasch,
S.G.Withers,
and
P.M.Alzari
(2004).
Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase.
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Structure, 12,
775-784.
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PDB codes:
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I.Kwon,
K.Ekino,
T.Oka,
M.Goto,
and
K.Furukawa
(2002).
Effects of amino acid alterations on the transglycosylation reaction of endoglucanase I from Trichoderma viride HK-75.
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Biosci Biotechnol Biochem, 66,
110-116.
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J.Karlsson,
D.Momcilovic,
B.Wittgren,
M.Schülein,
F.Tjerneld,
and
G.Brinkmalm
(2002).
Enzymatic degradation of carboxymethyl cellulose hydrolyzed by the endoglucanases Cel5A, Cel7B, and Cel45A from Humicola insolens and Cel7B, Cel12A and Cel45Acore from Trichoderma reesei.
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Biopolymers, 63,
32-40.
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C.Boisset,
C.Pétrequin,
H.Chanzy,
B.Henrissat,
and
M.Schülein
(2001).
Optimized mixtures of recombinant Humicola insolens cellulases for the biodegradation of crystalline cellulose.
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Biotechnol Bioeng, 72,
339-345.
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S.Fort,
A.Varrot,
M.Schülein,
S.Cottaz,
H.Driguez,
and
G.J.Davies
(2001).
Mixed-linkage cellooligosaccharides: a new class of glycoside hydrolase inhibitors.
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Chembiochem, 2,
319-325.
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PDB code:
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W.A.Breyer,
and
B.W.Matthews
(2001).
A structural basis for processivity.
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Protein Sci, 10,
1699-1711.
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R.Maheshwari,
G.Bharadwaj,
and
M.K.Bhat
(2000).
Thermophilic fungi: their physiology and enzymes.
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Microbiol Mol Biol Rev, 64,
461-488.
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A.Varrot,
M.Schülein,
and
G.J.Davies
(1999).
Structural changes of the active site tunnel of Humicola insolens cellobiohydrolase, Cel6A, upon oligosaccharide binding.
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Biochemistry, 38,
8884-8891.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
