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571 a.a.
_NI
Key reference
DOI no: 10.1016/j.jmb.2007.02.083 J Mol Biol 368:1215-1222 (2007) PubMed id: 17397864
The structural basis for exopolygalacturonase activity in a family 28 glycoside hydrolase. D.W.Abbott, A.B.Boraston. ABSTRACT
Family 28 glycoside hydrolases (polygalacturonases) are found in organisms across the plant, fungal and bacterial kingdoms, where they are central to diverse biological functions such as fruit ripening, biomass recycling and plant pathogenesis. The structures of several polygalacturonases have been reported; however, all of these enzymes utilize an endo-mode of digestion, which generates a spectrum of oligosaccharide products with varying degrees of polymerization. The structure of a complementary exo-acting polygalacturonase and an accompanying explanation of the molecular determinants for its specialized activity have been noticeably lacking. We present the structure of an exopolygalacturonase from Yersinia enterocolitica, YeGH28 in a native form (solved to 2.19 A resolution) and a digalacturonic acid product complex (solved to 2.10 A resolution). The activity of YeGH28 is due to inserted stretches of amino acid residues that transform the active site from the open-ended channel observed in the endopolygalacturonases to a closed pocket that restricts the enzyme to the exclusive attack of the non-reducing end of oligogalacturonide substrates. In addition, YeGH28 possesses a fused FN3 domain with unknown function, the first such structure described in pectin active enzymes.
Selected figure(s)
Figure 1.
Figure 2.
The above figures are reprinted by permission from Elsevier: J Mol Biol (2007, 368, 1215-1222) copyright 2007. Figures were selected by an automated process.
Literature references that cite this PDB file's key reference
PubMed id Reference
18430740 C.Creze, S.Castang, E.Derivery, R.Haser, N.Hugouvieux-Cotte-Pattat, V.E.Shevchik, and P.Gouet (2008).
The crystal structure of pectate lyase peli from soft rot pathogen Erwinia chrysanthemi in complex with its substrate.J Biol Chem, 283, 18260-18268.
PDB codes: 3b4n 3b8y 3b90
18535148 D.W.Abbott, and A.B.Boraston (2008).
Structural biology of pectin degradation by Enterobacteriaceae.Microbiol Mol Biol Rev, 72, 301.
18704748 Z.Xiao, S.Wang, H.Bergeron, J.Zhang, and P.C.Lau (2008).
A flax-retting endopolygalacturonase-encoding gene from Rhizopus oryzae.Antonie Van Leeuwenhoek, 94, 563-571. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.
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