PDBsum entry: 2oyl

Hydrolase

PDB-id: 2oyl

Main view

Contents

Description

Header details

Header records

References

PROCHECK

Protein chains

434 a.a. *

Ligands

IDC ×2

GOL ×2

Metal ions

_NA ×4

Waters ×849

* Residue conservation analysis

Tools

Clefts Calculation

Bottom view	Right view

PDB id:

2oyl

Name:

Hydrolase

Title:

Endo-glycoceramidase ii from rhodococcus sp.: Cellobiose- like imidazole complex

Structure:

Endoglycoceramidase ii. Chain: a, b. Engineered: yes

Source:

Rhodococcus sp.. Organism_taxid: 1831. Strain: m-777. Expressed in: escherichia coli. Expression_system_taxid: 562.

UniProt:

Chains A, B: O33853 (O33853_RHOSO)

Seq:

Struc:

Seq:

490 a.a.

Struc:

434 a.a.

Key:

PfamA domain

Secondary structure

Enzyme class:

E.C.3.2.1.123   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Oligoglycosylglucosylceramide + H₂O = ceramide + oligoglycosylglucose (see diagram below)

Resolution:

1.80Å

R-factor:

0.175

R-free:

0.205

Authors:

M.E.C.Caines,N.C.J.Strynadka

Key ref:

M.E.Caines et al. (2007). The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system.. Angew Chem Int Ed Engl, 46, 4474-4476. [PubMed id: 17487923] [DOI: 10.1002/anie.200700268]

Date:

22-Feb-07

Release date:

06-Mar-07

Quick_links

Procheck

Clefts

Surface

Key reference

DOI no: 10.1002/anie.200700268

Angew Chem Int Ed Engl 46:4474-4476 (2007)

PubMed id: 17487923

The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system.

M.E.Caines, S.M.Hancock, C.A.Tarling, T.M.Wrodnigg, R.V.Stick, A.E.Stütz, A.Vasella, S.G.Withers, N.C.Strynadka.

ABSTRACT

No abstract given.